天麻素对颈性高血压病模型兔椎动脉血流速度、血清去甲肾上腺素、血管紧张素Ⅱ和醛固酮含量的影响  

Effects of gastrodin onEnd-Diastolic VelocityofVertebral Artery andContents of Serum Norepinephrine,AngiotensinⅡand Aldosterone in Rabbits with Cervical Hypertension

作　　者：刘芳[1] 谢思维 方浩亮 黄哲女 顾逸文 LIU Fang;XIE Siwei;FANG Haoliang;HUANG Zhe′nyu;GU Yiwen(Department of Acupuncture,Zhejiang Hospital of Integrated Traditional Chinese and Western Medicine,Hangzhou310003,Zhejiang,China;The Second Clinical Medical College,Zhejiang Chinese Medical University,Hangzhou310053,Zhejiang,China;Department of Acupuncture,Fenghua District Hospitalof Traditional Chinese Medicine,Ningbo315500,Zhejiang,China)

机构地区：[1]浙江省中西医结合医院针灸科,浙江杭州310003 [2]浙江中医药大学第二临床医学院,浙江杭州310053 [3]宁波市奉化区中医医院针灸推拿康复科,浙江宁波315500

出　　处：《辽宁中医杂志》2024年第11期198-201,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81704144);国家中医药管理局第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]1号);浙江省中医药科技计划项目(2020ZB190);浙江省卫生高层次人才培养工程项目(浙卫办[2020]18号)。

摘　　要：目的观察天麻素对颈性高血压病模型兔椎动脉内径(vertebral arterial diameter,VD)、舒张末期最大血流速度(maximum end diastolic velocity,EDV)及血清中去甲肾上腺素(norepinephrine,NE)、血管紧张素Ⅱ(angiotensin,AngⅡ)和醛固酮(aldosterone,ALD)含量变化,探讨天麻素的可能作用机制。方法建立颈性高血压病家兔模型,将造模成功的12只实验兔按随机数字表法分为模型组(6只)和天麻素组(6只)。模型组耳缘静脉注射0.9%氯化钠溶液2 mL/(kg·d),天麻素组耳缘静脉注射含有50 mg/mL天麻素的等量0.9%氯化钠溶液,与6只正常对照组比较。观察并记录各组实验兔治疗前后椎动脉VD和EDV值,并运用酶联免疫吸附试验技术测定实验兔血清中NE、AngⅡ和ALD水平。结果与该组治疗前比较,模型组和天麻素组实验兔治疗后椎动脉内径、EDV值差异无统计学意义(P>0.05);与正常对照组比较,模型组和天麻素组实验兔治疗后椎动脉内径、EDV值差异无统计学意义(P>0.05)。与正常对照组比较,模型组NE水平(32.34±12.49)ng/mg,高于正常对照组(19.29±4.48)ng/mg(P<0.05),天麻素组与正常对照组比较差异无统计学意义(P>0.05)。模型组和天麻素组AngⅡ值与正常对照组比较,差异无统计学意义(P>0.05);模型组ALD值(160.07±38.14)pg/mg,高于正常对照组(103.33±29.83)pg/mg(P<0.01),而天麻素组ALD值与正常对照组比较,差异无统计学意义(P>0.05)。结论天麻素对颈性高血压病具有良好的改善作用,其机制可能与改善代谢、抑制交感神经兴奋及抑制醛固酮的表达有关。Objective To observe the effect of gastrodin on the vertebral arterial diameter(VD),maximum end diastolic velocity(EDV),and serum contentsof norepinephrine(NE),angiotensinⅡ(AngⅡ)and aldosterone(ALD)of rabbits with cervical hypertension,and to explore its possible action mechanism.Methods A cervical hypertension rabbit model was established.Twelve experimental rabbits were equally divided among the model and gastrodin groups according to the random number table.The model group was injected with 2 mL/(kg·d)of normal saline in the ear′s edge vein,while the gastrodin group was injected with the normal saline containing 50 mg/mL gastrodin.Then the values of VD and EDV of the vertebral artery were recorded before and after the treatment,and the serum levels of NE,AngⅡand ALD in the experimental rabbits were measured by using enzyme-linked immunosorbent assay(ELISA).Results Compared with those before treatment,there was no statistical difference inthe values of VD or EDV of the model and gastrodin groups after treatment(P>0.05).Compared with those of the normal control group,there was no statistically significant difference in the vertebral artery diameter and EDV values of the model group and the gastrodin group after treatment(P>0.05).Compared with that in the normal control group,the NE level in the model group[(32.34±12.49)ng/mg]was higher than that in the normal control group[(19.29±4.48)ng/mg](P<0.05),and there was no statistically significant difference between the gastrodin group and the normal control group(P>0.05).There was no statistically significant difference in AngⅡvalues between the model group and the gastrodin group compared to those in the normal control group(P>0.05).The ALD value of the model group[(160.07±38.14)pg/mg]was higher than that of the normal control group[(103.33±29.83)pg/mg](P<0.01),while there was no statistically significant difference in ALD value between the gastrodin group and the normal control group(P>0.05).Conclusion Gastrodin has a good effect on the improvement o

关 键 词：天麻素 颈性高血压病 椎动脉 RAAS系统 

分 类 号：R259.441[医药卫生—中西医结合]