茄子反向温敏雄性不育系内源激素含量及相关基因表达分析  

Analysis of endogenous hormones content and related gene expression in reverse thermo-sensitive genic male sterile line of eggplant

作　　者：张敬敬[1] 李冰[1] 高秀瑞[1] 田鹏 刘伟 赵鑫泽 刘会茹 潘秀清[1] 武彦荣[1] ZHANG Jingjing;LI Bing;GAO Xiurui;TIAN Peng;LIU Wei;ZHAO Xinze;LIU Huiru;PAN Xiu-qing;WU Yanrong(Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,Hebei,China)

机构地区：[1]河北省农林科学院经济作物研究所,石家庄050051

出　　处：《中国瓜菜》2024年第11期27-37,共11页China Cucurbits And Vegetables

基　　金：河北省自然科学基金项目(C2023301109);河北省省级科技计划资助项目(21326309D);河北省露地蔬菜创新团队项目(HBCT2024140202);河北省农林科学院现代农业科技创新专项课题资助项目(2022KJCXZX-JZS-2)。

摘　　要：雄性不育对杂交育种至关重要,挖掘茄子雄性不育关键候选基因有助于定向改良不育材料。以长茄温敏型雄性不育系05ms及其同源可育系S63为材料,对不育系05ms和可育系S63减数分裂时期(Ⅱ)的花药进行转录组测序,并对05ms春季低温不育(ML)和夏季高温可育(MH)阶段的花粉母细胞时期(Ⅰ)、减数分裂期(Ⅱ)、小孢子时期(Ⅲ)和成熟花粉粒(Ⅳ)4个花药发育时期的内源激素含量进行检测。结果表明,差异表达基因主要富集在戊糖和葡萄糖酸酯的转化、类胡萝卜素的生物合成和植物激素信号转导等代谢通路,Smechr0702283、Smechr0801802、Smechr0600105、Smechr0802213、Smechr0302844、Smechr0601316、Smechr0401804等基因为茄子温敏雄性不育的关键调控基因,实时荧光定量PCR(qRT-PCR)结果表明,转录组数据可靠。激素代谢物检测结果表明,生长素类激素ICAld和乙烯前体ACC含量在4个时期中ML均显著高于MH,而ML的生长素IAA含量低于MH。从代谢水平和转录水平解析了茄子花药的败育机制,研究结果为进一步揭示茄子温敏雄性不育调控机制奠定了理论基础。Male sterility is crucial for hybrid breeding,and digging key candidate genes for male sterility of eggplant is helpful to directional improvement of male sterile materials.In this experiment,using the thermo-sensitive male sterile line 05ms and the fertile line S63 as experimental materials,transcriptome sequencing was performed on the anthers of sterile line 05ms and fertile line S63 at meiosis stage(II),and the endogenous hormone contents of the pollen mother cell stage(I),meiosis stage(II),microspore stage(III)and mature pollen grain stage(IV)of 05ms under low-temperature sterility period(ML)in spring and high-temperature fertility period(MH)in summer were detected.The results showed that differential expressed genes were mainly concentrated in the metabolic pathways of pentose and gluconate conver-sion,carotenoid biosynthesis and plant hormone signal transduction.The key regulatory genes of thermo-sensitive male sterility in eggplant,such as Smechr0702283,Smechr0801802,Smechr0600105,Smechr0802213,Smechr0302844,Smechr0601316,Smechr0401804,were identified by qRT-PCR,which confirmed the reiliability of the transcriptome data.The results of hormone metabolite detection showed that the content of auxin hormone ICAld and ethylene precursor ACC in ML were significantly higher than those in MH at all of the four stages,while the content of auxin IAA in ML were lower than those in MH.The author analyzed the mechanism of anther abortion in eggplant at the metabolism and transcription level,which laid a theoretical foundation for further revealing the regulatory mechanism of thermo-sensitive male sterility in eggplant.

关 键 词：茄子 转录组 代谢组 温敏雄性不育 激素 

分 类 号：S641.1[农业科学—蔬菜学]