藻粉对宽体金线蛭生长、酶活性和基因表达的影响  

Alga Meals Affect Growth,Enzyme Activities,and Gene Expression of Leech Whitmania pigra

作　　者：闫晶男 吴怡蓉 张雨婷 江玲丽[2] 高有领[1] YAN Jingnan;WU Yirong;ZHANG Yuting;JIANG Lingli;GAO Youling(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China;Ningbo College of Health Sciences,Ningbo 315100,China)

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100 [2]宁波卫生职业技术学院,浙江宁波315100

出　　处：《水产科学》2024年第6期974-983,共10页Fisheries Science

基　　金：浙江省科技厅研究项目(LGN22C190028);宁波市科技局项目(202002N3048)。

摘　　要：为揭示方形环棱螺搭配投喂蛋白核小球藻粉或螺旋藻粉对宽体金线蛭生长、消化酶活性、抗凝血酶活性和相关基因表达的影响,将体质量(4.15±0.03)g的宽体金线蛭随机放入38.5 cm×27.5 cm×13.5 cm的透明塑料盒中,每盒20条,分别投喂方形环棱螺(螺组)、方形环棱螺加蛋白核小球藻粉(小球藻粉组)和方形环棱螺加螺旋藻粉(螺旋藻粉组)30 d,螺每1 d、藻粉每2 d投喂1次,养殖30 d时,测定生长激素(GH)、胰岛素样生长因子(IGF1)等生长相关基因的表达量,α-葡萄糖苷酶(α-GLU)、甘油三酯酶(ATGL)和蛋白酶(PT)消化酶相关基因的表达量,及免疫相关基因超氧化物歧化酶(SOD)和水蛭素(WP)基因的表达量。结果表明:10、20 d和30 d小球藻粉组宽体金线蛭的体质量高于其他组(P<0.05),10 d的特定生长率高于螺组(P<0.05),25 d和30 d的高于螺旋藻粉组(P<0.05),30 d的摄食量高于其他两组(P<0.05),饲料系数在10 d优于螺组,且在30 d优于螺旋藻粉组(P<0.05);养殖30 d后,各组存活率在91.67%以上;小球藻粉组和螺旋藻粉组抗凝血酶活性在30 d均高于螺组(P<0.05),小球藻粉组的脂肪酶活性高于螺组和螺旋藻粉组(P<0.05);小球藻粉组宽体金线蛭的GH、IGF1和PT基因表达量在30 d高于螺旋藻粉组且小球藻粉组的GH和PT基因表达量高于螺组(P<0.05)。综上所述,蛋白核小球藻粉作为辅助饵料与方形环棱螺搭配投喂宽体金线蛭30 d,显著促进了生长,提高了脂肪酶和抗凝血酶的活性,影响了相关基因的表达。In order to reveal the effects of alga Chlorella pyrenoidosa and Spirulina platensis meals as assistant diet on the growth,digestive enzyme and antithrombin activities,as well as related gene expression of leech Whitmania pigra,leeches with body weight of(4.15±0.03)g were randomly placed into transparent plastic boxes of each 8.5 cm×27.5 cm×13.5 cm at density of 20 individuals per box,and were fed snail S.quadrata(snail group)once a day,the snail+C.pyrenoidosa powder(chlorella powder group)and the snail+S.platensis(spirulina powder group)once every other day with triplicate for 30 days.The digestive tract tissues were dissected,frozen with liquid nitrogen,and the expressions levels of growth-related genes such as growth hormone(GH)and insulin-like growth factor-1(IGF1),and expression levels ofαα-glucosidase(α-GLU),adipose triglyceride lipase(ATGL),protease(PT)digestive enzyme-related genes,and expression levels of immune-related genes superoxide dismutase(SOD)and hirudin gene(WP)were detected at the end of the experiment by MyiQ2 Two Color real-time quantitative PCR(Bio-Rad).The results showed that there was significantly higher body weight in chlorella powder group(P<0.05)in 10 d,20 d and 30 d,significantly higher specific growth rate than that in snail group in 10 d,and significantly higher than that in spirulina powder group in 25 d and 30 d(P<0.05).The leech had significantly higher food intake in chlorella powder group than that in other groups in 30 d(P<0.05),with significantly lower food conversion ratio than that in snail group in 10 d,and in spirulina powder group in 30 d(P<0.05).The survival rate was found to be over 91.67%in all groups.Moreover,significantly higher antithrombin activity was observed in spirulina powder group and chlorella powder group than that in snail group in 30 d(P<0.05),with significantly higher lipase activity in chlorella powder group than that in the other groups in 30 d(P<0.05).There were significantly higher expression levels of GH,IGF1 and PT genes in chlorella powder

关 键 词：宽体金线蛭 蛋白核小球藻粉 螺旋藻粉 生长 消化酶 抗凝血酶 

分 类 号：S963.2[农业科学—水产养殖]