生长调节剂对自然低温下红海榄幼苗生理特性的影响  

Effects of Growth Regulators on Physiological Characteristics of Rhizophora stylosa Seedlings under Natural Low Temperature

作　　者：贾湘璐 欧阳子龙 石景忠 滕维超[4] 樊东函 刘秀[1] JIA Xianglu;OUYANG Zilong;SHI Jingzhong;TENG Weichao;FAN Donghan;LIU Xiu(Guangxi Forestry Research Institute,Nanning,Guangxi 530002,China;Nanning Botanical Garden,Nanning,Guangxi 530002,China;Nanning Qingxiu Mountain Scenic Spots Tourism Development Co.,Ltd.,Nanning,Guangxi 530004,China;College of Forestry,Guangxi University,Nanning,Guangxi 530004,China;Qinzhou Forestry Research Institute,Qinzhou,Guangxi 535099,China)

机构地区：[1]广西壮族自治区林业科学研究院,广西南宁530002 [2]南宁植物园,广西南宁530002 [3]南宁青秀山风景名胜旅游开发有限责任公司,广西南宁530004 [4]广西大学,广西南宁530004 [5]钦州市林业科学研究所,广西钦州535099

出　　处：《林业与环境科学》2024年第5期27-38,共12页Forestry and Environmental Science

基　　金：广西重点研发计划项目(桂科AB24010288);广西壮族自治区林业科学研究院团队项目(2024RT27);广西林业科技推广示范项目(桂林科研[2021]1号)。

摘　　要：为探究生长调节剂对冬季自然低温条件下红海榄Rhizophora stylosa幼苗生理特性的影响,并为其幼苗繁殖保育工作提供理论依据和指导,研究以红海榄2个月实生苗为试验材料,选择6-BA、NAA、GA_(3)、IAA共4种生长调节剂进行试验处理,测定叶片光合色素、丙二醛(MDA)和抗氧化酶活性指标,进行相关性和主成分分析。结果表明,自然低温下连续喷施1~3个月不同种类和浓度的生长调节剂均能有效提高红海榄幼苗CHLa、CHLb、CHLa+b和CC质量分数并降低MDA质量摩尔浓度;SOD、POD、CAT和APX活性则随时间波动变化。相关性分析表明,红海榄各生理指标间存在一定的协同作用,且抗氧化酶活性、光合色素均与MDA呈负相关。主成分综合得分排名为150 mg·L^(-1)IAA>50 mg·L^(-1)NAA>100 mg·L^(-1)NAA>100 mg·L^(-1)IAA>400 mg·L^(-1)GA_(3)>400 mg·L^(-1)6-BA>150 mg·L^(-1)NAA>200 mg·L^(-1)6-BA>200 mg·L^(-1)GA_(3)>100 mg·L^(-1)GA_(3)>50 mg·L^(-1)IAA>100 mg·L^(-1)6-BA>CK。生长调节剂能有效改善自然低温下红海榄幼苗的生理状态,其中150 mg·L^(-1)IAA处理效果最佳。To investigate the effects of plant growth regulators on physiological characteristics of Rhizophora stylosa seedlings under natural low temperature in winter,and to provide a theoretical basis and guidance for the reproduction and conservation of R.stylosa seedlings,four growth regulators,6-BA,NAA,GA_(3) and IAA,were selected as experimental materials to determine the photosynthetic pigment,MDA content and antioxidant enzyme activity indexes in the leaves of 2 months R.stylosa seedlings.The correlation among the indexes was calculated and the comprehensive score ranking was calculated by principal component analysis.The results showed that the concentrations of CHLa,CHLb,CHLa+B,and CC were increased and the concentrations of MDA were decreased by spraying different kinds and concentrations of plant growth regulators for 1 to 3 months under the natural low temperature condition.The activity of SOD,POD,CAT and APX fluctuated with time.Correlation analysis showed that there was a certain synergistic effect among the physiological indexes of R.stylosa,and the antioxidant enzyme activities and photosynthetic pigments were negatively correlated with MDA.The overall score of principal component was 150 mg·L^(-1) IAA>50 mg·L^(-1) NAA>100 mg·L^(-1) NAA>100 mg·L^(-1) IAA>400 mg·L^(-1) GA_(3)>400 mg·L^(-1)6-BA>150 mg·L^(-1) NAA>200 mg·L^(-1)6-BA>200 mg·L^(-1) GA_(3)>100 mg·L^(-1) GA_(3)>50 mg·L^(-1) IAA>100 mg·L^(-1)6-BA>CK.Plant growth regulators can effectively improve the physiological state of R.stylosa under natural low temperature,and 150 mg·L^(-1) IAA treatment has the best effect.

关 键 词：生长调节剂 红海榄 自然低温 幼苗 生理特性 

分 类 号：S718.43[农业科学—林学]