河南省山药病毒病病原鉴定及主要病毒的序列分析  

Pathogen identification of yam virus disease in Henan Province and sequence analysis of main viruses

作　　者：秦艳红[1] 鲁书豪 王凤丽 刘玉霞[1] 文艺[1] 高素霞[1] 李绍建[1] 吴绪金 王飞[1] 鲁传涛[1] QIN Yanhong;LU Shuhao;WANG Fengli;LIU Yuxia;WEN Yi;GAO Suxia;LI Shaojian;WU Xujin;WANG Fei;LU Chuantao(Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Institute of Agricultural Quality Standards and Detection Technology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]河南省农业科学院农业质量标准与检测技术研究所,郑州450002

出　　处：《植物病理学报》2024年第5期913-924,共12页Acta Phytopathologica Sinica

基　　金：国家中药材产业技术体系(CARS-B21);河南省中药材产业技术体系(HARS-22-11-Z1);河南省农业科学院基本科研业务费项目(2023ZC051)。

摘　　要：为开展河南省山药病毒种类及遗传变异的系统性研究,通过高通量测序(high-throughput sequencing)和RT-PCR检测疑似病毒病的山药样品188份,结果表明从山药样品中检测出5种病毒:日本山药花叶病毒(Japanese yam mosaic virus,JYMV)、油菜花叶病毒(youcai mosaic virus,YoMV)、山药潜隐病毒(yam latent virus,YLV)、蚕豆萎蔫病毒2(broad bean wilt virus 2,BBWV 2)和淮山药黄斑花叶病毒(yam yellow spot mosaic virus,YYSMV)。JYMV、YoMV、YYSMV、BBWV 2和YLV的检出率分别为94.15%、87.23%、68.09%、42.02%和29.79%,其中98.94%的山药样品都存在复合侵染现象。本研究统计188份样品中共有20种复合侵染类型,其中JYMV+YYSMV+YoMV是主要复合侵染类型,检出率是26.60%。序列分析结果表明,YoMV和JYMV较保守,其次是YYSMV,YLV和BBWV 2变异较大,遗传进化树分析结果表明本研究获得的分离物与同一地区的分离物亲缘关系较近。同时HTS分析结果表明在山药上检测到其他未明确分类地位的病毒种类,因此后续还需全面系统地对河南省山药病毒的种类开展研究。To study the species and genetic variation of yam viruses in Henan province,188 yam samples suspected of viral diseases were analyzed by high-throughput sequencing and RT-PCR.The results showed that five viruses were detected from yam samples:Japanese yam mosaic virus(JYMV),youcai mosaic virus(YoMV),yam latent virus(YLV),broad bean wilt virus 2(BBWV 2)and yam yellow spot mosaic virus(YYSMV).The detection rates of JYMV,YoMV,YYSMV,BBWV 2 and YLV were 94.15%,87.23%,68.09%,42.02%and 29.79%,respectively.98.94%of the yam samples had complex infection.In this study,there were 20 complex infection types in 188 samples,among which JYMV+YYSMV+YoMV was the main complex infection type,and the detection rate was 26.60%.Molecular variation analysis showed that YoMV and JYMV were more highly conserved,followed by YYSMV,YLV and BBWV 2,which showed more variation.Phylogenetic tree analyses indicated that the isolates obtained in this study were closely related to those from the same region.At the same time,HTS analysis results showed that other unclassified virus species were detected on yam,so it is necessary to conduct a comprehensive and systematic study on the types of yam viruses in Henan Province.

关 键 词：山药病毒病 高通量测序 病原鉴定 复合侵染 序列分析 

分 类 号：S432.41[农业科学—植物病理学]