贵州黔西南烟草中烟草脉带花叶病毒的鉴定及遗传结构分析  

Identification and genetic structure analysis of tobacco vein banding mosaic virus isolate from tobacco plants in Guizhou Qianxinan Prefecture

作　　者：陈越[1] 穆青 李婷婷[1] 罗富方 刘朝忠 曹毅 丁铭[1] CHEN Yue;MU Qing;LI Tingting;LUO Fufang;LIU Chaozhong;CAO Yi;DING Ming(Yunnan Province Key Laboratory of Agricultural Biotechnology,Key Lab of the Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture,Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China;Guizhou Qianxinan Prefectural Tobacco Company,Xingyi 562400,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业部西南农业基因资源与种质创制重点实验室,昆明650205 [2]贵州省烟草科学研究院,贵阳550081 [3]贵州省烟草公司黔西南州公司,兴义562400

出　　处：《植物病理学报》2024年第5期925-936,共12页Acta Phytopathologica Sinica

基　　金：贵州省烟草公司黔西南州公司科技项目(2022-01)。

摘　　要：为了明确贵州省黔西南州烟草中烟草脉带花叶病毒(tobacco vein banding mosaic virus,TVBMV)的发生和病毒的群体遗传结构,了解该TVBMV贵州烟草分离物上的遗传变异机制。利用电子显微镜负染色、超薄切片制样观察及分子生物学方法对采集自贵州省黔西南州兴义市具有典型TVBMV症状的烟草样品进行检测和鉴定,并利用生物信息学软件对其进行基于病毒分离物CP基因序列的系统进化和群体遗传结构分析。结果表明:采集的22份烟草样品中,TVBMV的检出率为13.64%;所获得的TVBMV贵州烟草分离物与GenBank上公布的其它42个分离物CP基因的核苷酸和氨基酸序列的一致性分别为94.1%~99.6%和93.4%~100%;基于CP氨基酸序列的系统发育分析按照地理分布将分离物划分为4个类群,聚类结果具有明显的地理分布特征;遗传多样性分析显示,受到地理分布的影响,各组TVBMV分离物均表现出较高的遗传多样性水平;种群遗传结构分析发现,负选择作用、遗传漂变和生态环境是TVBMV种群遗传变异的重要方式。研究结果为贵州烟草病毒病的防控和抗病育种提供一定的理论支持。In order to investigate the occurrence and population genetic structure of tobacco vein banding mosaic virus on tobacco in Xingyi City,Guizhou Qianxinan Prefecture,and understand the genetic variation mechanism of tobacco vein banding mosaic viruses on tobacco isolates in Guizhou,Detection and identification of tobacco samples with typical TVBMV symptoms collected from Xingyi City,Qianxinan Prefecture,Guizhou Province were performed using electron microscopy negative staining,ultra-thin section preparation observation and molecular biology methods.Phylogenetic analysis and population genetic structure analysis of Guizhou tobacco samples was based on CP gene sequence of virus isolates using bioinformatics software.The results showed that among the 22 tobacco samples collected,the detection rate of TVBMV was 13.64%.The consistencies of nucleotide and amino acid sequences between the obtained TVBMV Guizhou tobacco isolate and the other 42 isolates published in GenBank were 94.1%-99.6%and 93.4%-100%,respectively.Phylogenetic analysis based on the amino acid sequence of the CP gene divides the isolates into four groups corresponding to the geographical distribution.The clustering results have obvious geographical distribution characteristics.Genetic diversity analysis shows that each group of TVBMV isolates exhibits a high level of genetic diversity due to the influence of geographical distribution.The analysis of population genetic structure shows that negative selection,genetic drift and ecological environment are the important effoectors for the genetic variation of TVBMV population.The research results provide theoretical support for the prevention and control of tobacco virus diseases and disease resistance breeding in Guizhou.

关 键 词：烟草 烟草脉带花叶病毒 群体遗传结构 遗传变异 CP基因 

分 类 号：S432.1[农业科学—植物病理学]