HMG-box家族基因MfHMG5和MfHMG6对桃褐腐病菌生长、产孢和致病的影响  

Functions of HMG-box family genes MfHMG5 and MfHMG6 on growth,sporulation and pathogenicity in Monilinia fructicola

作　　者：杨静雅 曾哲政 肖媛玲 蔡民政 吴沛珊 魏闻恺 阴伟晓 罗朝喜[1,2] YANG Jingya;ZENG Zhezheng;XIAO Yuanling;CAI Minzheng;WU Peishan;WEI Wenkai;YIN Weixiao;LUO Chaoxi(National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,Huazhong Agricultural University,Wuhan 430070,China;Hubei Key Lab of Plant Pathology and College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学果蔬园艺作物种质创新与利用全国重点实验室,武汉430070 [2]华中农业大学植物科技学院湖北省作物病害监测及安全控制重点实验室,武汉430070

出　　处：《植物病理学报》2024年第5期961-973,共13页Acta Phytopathologica Sinica

基　　金：国家桃产业技术体系(CARS-30);国家自然科学基金(31371896)。

摘　　要：由链核盘菌引起的褐腐病严重危害核果和仁果类水果的生产,极大影响我国水果的远距离销售与出口。基于对实生链核盘菌(Monilinia fructicola)基因组学和侵染早期转录组学分析,筛选到MfHMG5和MfHMG6基因在桃褐腐病菌侵染早期各时间段的表达量变化趋势相同,都在侵染1 h后显著下降,而后逐渐上升。为阐明两者的生物学功能,对MfHMG5的敲除和过表达转化子,MfHMG6的敲除和回补转化子的表型进行观察,发现MfHMG5基因的缺失和过量表达都降低褐腐病菌的生长速率和产孢能力,但不影响其致病力。MfHMG6基因的缺失降低了褐腐病菌的生长速率、致病力和产孢能力,且导致MfHMG5基因的表达量升高。本研究结果说明MfHMG5和MfHMG6参与调控桃褐腐病菌的生长与致病。Brown rot,caused by Monilinia spp.,is a serious threat to both stone fruit and pome fruit,greatly affecting the long-distance transportation and exportation of fruits.Based on genomic and transcriptomic analysis of infection of Monilinia fructicola on peach fruit,it was detected that the expression patterns of MfHMG5 and MfHMG6 genes in early stages of infection were similar,and both down-regulated significantly at 1 h after inoculation and then gradually increased.In order to investigate the biological functions of these two genes,the knockout and overexpression transformants of MfHMG5,and knockout and complemented transformants of MfHMG6 were obtained and the corresponding phenotypes were investigated.It was found that the knockout and overexpression of MfHMG5 gene decreased the growth rate and sporulation ability,but did not affect the pathogenicity of M.fructicola.Knock out of MfHMG6 gene reduced the growth rate,virulence and sporulation ability of M.fructicola,and led to the increased expression of MfHMG5 gene.These results indicated that HMG-box family genes MfHMG5 and MfHMG6 were involved in regulating the growth and pathogenesis of M.fructicola.

关 键 词：桃褐腐病 实生链核盘菌 HMG-BOX 产孢 致病机制 

分 类 号：S432.44[农业科学—植物病理学]