甜樱间座壳菌(Diaporthe eres)引起苹果根颈腐烂病研究简报  

Brief report of apple diaporthe neck and root rot caused by Diaporthe eres

作　　者：莫艳芳 普特 罗强 施竹凤 杨童雨 矣小鹏 申云鑫 王楠 廖永琴 何永宏[1] 陈齐斌[1] 杨佩文[2] MO Yanfang;PU Te;LUO Qiang;SHI Zhufeng;YANG Tongyu;YI Xiaopeng;SHEN Yunxin;WANG Nan;LIAO Yongqin;HE Yonghong;CHEN Qibin;YANG Peiwen(College of Plant Protection,Yunnan Agricultural University,Yunnan 650201,China;Institute of Agricultural Environment and Resources,Yunnan Academy of Agricultural Sciences,Yunnan 650205,China)

机构地区：[1]云南农业大学植物保护学院,云南650201 [2]云南省农业科学院农业环境与资源研究所,云南652025

出　　处：《植物病理学报》2024年第5期1036-1041,共6页Acta Phytopathologica Sinica

基　　金：云南省科技计划重大科技专项(202202AE090029);国家自然科学基金(32060624)。

摘　　要：随着云南高原特色农业各项工作的深入实施,苹果产业成为区域农业产业的重要支撑,是广大山区半山区农民脱贫致富可靠的经济创收途径。另一方面,根腐病是近年来在苹果主产区发生最广泛、危害最严重的病害之一,发病率一般在10%左右,严重的达50%以上[1]。In recent years,apple diaporthe neck and root rot has become one of the most important limiting factors for the development of apple production in Yunnan Province.In this study,the typical disease samples were collected and the fungal isolate M2g7-1 was obtained by tissue separation approach.The purified culture M2g7-1 was preliminary determined belonging to Diaporthe spp.based on the morphological features of colony,pycnidia,cirrus,two forms of conidia.The pathogenicity of M2g7-1 was further validated on apple young branches to fulfill the Koch's law.The taxonomic of pathogen M2g7-1 has been further determined with combining molecular phylogenetics.The phylogenetic tree was created with the data set of sequences from the internal transcribed spacer(ITS)of ribosomal DNA,the histone H3(HIS)gene,the translation elongation factor 1-alpha(TEF)gene,and the beta-tubulin(TUB)gene.Based on morphological and molecular biology analysis,the pathogen M2g7-1 was finally identified as Diaporthe eres.This is the first report of D.eres as the pathogen of apple diaporthe neck and root rot.The research results provide a theoretical basis for the epidemic research and comprehensive control of this disease.

关 键 词：苹果主产区 苹果产业 根腐病 农民脱贫致富 区域农业产业 山区半山区 研究简报 

分 类 号：S432.44[农业科学—植物病理学]