侵染海南樱桃番茄的病毒种类鉴定  

Identification of viral pathogens from the cherry tomato in Hainan Province

作　　者：车海彦[1,2] 裴月令 孙燕芳[1,3] 陈园 罗大全[1,2] 龙海波 CHE Haiyan;PEI Yueling;SUN Yanfang;CHEN Yuan;LUO Daquan;LONG Haibo(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture and Rural Affairs,Haikou 571101,China;Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou 571101,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海口571101 [2]农业农村部热带作物有害生物综合治理重点实验室,海口571101 [3]海南省热带农业有害生物监测与控制重点实验室,海口571101

出　　处：《植物病理学报》2024年第5期1063-1068,共6页Acta Phytopathologica Sinica

基　　金：中国热带农业科学院基本科研业务费专项项目(1630042019001);2019年海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC284)。

摘　　要：樱桃番茄(Solanum lycopersicum var.cerasiforme)又名圣女果、小番茄、迷你番茄,为茄科(Solanaceae)茄属(Solanum)植物,由野生醋栗番茄(Solanum pimpinellifolium)驯化而来,也是普通大果番茄的祖先[1]。自1997年台商将樱桃番茄成功引入海南种植以来,樱桃番茄的种植面积和种植区域逐年扩大,至2018年,海南省种植面积达7822hm^(2),产量为28.15万吨,主要种植在陵水、昌江、定安等市县[2]。Cherry tomato(Solanum lycopersicum var.cerasiforme)is an important crop in Hainan Province,and its production is seriously damaged by viral diseases.To identify the viruses infecting cherry tomato,LncRNA sequencing was performed using leaf samples from diverse geographical locations with typical viral symptoms.The virome of cherry tomato comprises eight viruses─ageratum yellow vein virus(AYVV),cucumber mosaic virus(CMV),southern tomato virus(STV),tobacco mosaic virus(TMV),tomato chlorosis virus(ToCV),tomato mosaic virus(ToMV),tomato mottle mosaic virus(ToMMV)and tomato yellow leaf curl virus(TYLCV).The PCR detection results of 234 cherry tomato leaf samples from nine cities and counties indicated that the dominant viruses are TYLCV(69.23%),ToCV(49.57%)and ToMMV(35.47%),followed by STV(23.50%),ToMV(17.52%),CMV(17.52%),AYVV(16.24%)and TMV(10.68%).The ratio of mixed virus infection was 81.62%.Complete genomes of two AYVV isolates(AYVV-tomato-LS1 and AYVV-tomato-LS2)and one TYLCV isolate(TYLCV-tomato-LS)in the genus Begomovirus were obtained using PCR with back-to-back primers.The pairwise comparison of genomes of AYVV-tomato-LS1 and AYVV-tomato-LS2 were 98.98%nucleotide identical to each other,sharing their maximum nucleotide identities at 92.71%and 92.81%with AYVV-SY08(GenBank accession no.:KC810890),and were phylogenetically closely related to the AYVV isolates from diverse hosts from Hainan.TYLCV-tomato-LSs shared their maximum nucleotide identities at 96.52%with TYLCV-HNLS(GenBank accession no.:MK908814),and were phylogenetically closely related to the TYLCV isolates from diverse hosts from Hainan,followed by Capsicum annuum isolates from Spain(GenBank accession no.:AJ489258).Our findings provide support for sustainable control strategies for cherry tomato virus disease in Hainan,China.

关 键 词：樱桃番茄 小番茄 种植区域 大果番茄 醋栗 病毒种类鉴定 圣女果 

分 类 号：S432.44[农业科学—植物病理学]