南通市蚕豆赤斑病病原菌鉴定及其抑菌药剂筛选  

Identification and screening of antifungal agents for the pathogen causing broad bean chocolate spot in Nantong City

作　　者：王凡 卞晓春[1] 刘陈玮 徐仁超[1] 陆红臣 吴春芳[1] WANG Fan;BIAN Xiaochun;LIU Chenwei;XU Renchao;LU Hongchen;WU Chunfang(Jiangsu Yanjiang Institute of Agricultural Sciences,Nantong 226012,China)

机构地区：[1]江苏沿江地区农业科学研究所,江苏南通226012

出　　处：《江苏农业学报》2024年第10期1810-1817,共8页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省种业揭榜挂帅项目[JBGS(2021)058];江苏现代农业产业技术体系建设项目[JATS(2023)158];江苏沿江地区农业科学研究所青年科技基金项目[YJ(2022)009]。

摘　　要：为明确江苏省南通市蚕豆赤斑病的病原菌种类,对致病菌进行分离纯化,并结合致病性测定、形态学观察和多基因碱基序列分析对疑似病原菌进行鉴定。结果表明,南通市蚕豆赤斑病病原菌为拟蚕豆葡萄孢(Botrytis fabiopsis)。生物学特性分析结果表明,该病原菌菌丝的最适生长温度为25℃,最适光照条件为12 h光照+12 h黑暗,对pH不敏感,最适碳源为葡萄糖,最适氮源为酵母膏、牛肉膏和硫酸铵,最适培养基为马铃薯葡萄糖琼脂(PDA)培养基。抑菌药剂筛选结果表明,2.0%苦参碱水剂和10.0%苯醚甲环唑水分散粒剂对病原菌的抑制效果最好,半数效应浓度(EC_(50))分别为0.0054 mg/L和0.7554 mg/L,而10.0%多抗霉素可湿性粉剂和80.0%波尔多液可湿性粉剂对病原菌几乎无抑制效果。本研究结果可为江苏省南通市蚕豆赤斑病的科学防治提供理论基础。To clarify the pathogen causing broad bean chocolate spot in Nantong City,Jiangsu province,the pathogen was isolated and purified,and the suspected pathogen was identified through pathogenicity testing,morphological observation,and multi-gene base sequence analysis.The results indicated that the pathogen of broad bean chocolate spot in Nantong City was Botrytis fabiopsis.The results of biological characteristics analysis showed that the optimal growth temperature for the pathogen was 25℃,and the optimal light condition was 12 h light+12 h dark.The pathogen was insensitive to pH.The optimal carbon source for the pathogen was glucose,the optimal nitrogen sources were yeast extract,beef extract,and ammonium sulfate,and the optimal culture medium was potato dextrose agar(PDA)medium.The screening results of antifungal agents indicated that 2.0%sophocarpidine aqueous solution and 10.0%difenoconazole water dispersible granules had the best inhibitory effect on the pathogen,with median effective concentration(EC_(50))of 0.0054 mg/L and 0.7554 mg/L,respectively.However,10.0%polyoxin wettable powder and 80.0%Bordeaux liquid wettable powder had almost no inhibitory effect on the pathogen.The research results can provide a theoretical basis for the scientific prevention and control of broad bean chocolate spot in Nantong City,Jiangsu province.

关 键 词：蚕豆 赤斑病 病原鉴定 生物学特性 抑菌药剂 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]