金钗石斛多糖对产蛋后期鸡产蛋性能和盲肠微生物群落的影响  

Effects of Dendrobium nobile polysaccharides on laying performance and cecal microbial community of hens in late laying period

作　　者：谭启松 石钰仕 李辉[1] 龙霞 赵德鹏 余欢 陈友波 TAN Qisong;SHI Yushi;LI Hui;LONG Xia;ZHAO Depeng;YU Huan;CHEN Youbo(Key Laboratory of Aninal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education/College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]高原山地动物遗传育种与繁殖教育部重点实验室/贵州大学动物科学学院,贵州贵阳550025

出　　处：《江苏农业学报》2024年第10期1891-1903,共13页Jiangsu Journal of Agricultural Sciences

基　　金：贵州省科技厅农业领域重点项目[黔科合支撑(2022)重点034];贵州省教育厅项目[黔教技(2022)061号];贵州省农业农村厅项目[黔农计财(2022)10号]。

摘　　要：为研究金钗石斛多糖对产蛋后期隐性白羽鸡产蛋性能和肠道菌群的影响,饲喂白羽鸡金钗石斛多糖,饲喂期间对白羽鸡的产蛋指标进行记录。饲养60 d后,采集白羽鸡盲肠内容物用于细菌16S rRNA基因测序,并进行生物信息学分析。研究结果表明,与对照相比,金钗石斛多糖处理隐性白羽鸡的总产蛋数、产蛋量、日产蛋量和产蛋率极显著提高(P<0.01)。白羽鸡肠道微生物群落中,属水平上,优势菌属为拟杆菌属(Bacteroides)和里氏菌科Rc9肠道群(Rikenellaceae_Rc9_gut group);门水平上,优势菌门为拟杆菌门(Bacteroidota)和厚壁菌门(Fimicutes)。与对照相比,金钗石斛多糖处理瘤胃球菌群(Ruminococcus_torques_group)、粪球菌属(Faecalicoccus)与梅迪杆菌属(Merdibacter)等有益菌的相对丰度显著提升(P<0.05),巴恩斯氏菌属(Barnesiella)等致病菌的相对丰度显著降低(P<0.05)。表型预测结果显示,对照白羽鸡肠道微生物群落革兰氏阳性菌相对丰度显著低于金钗石斛多糖处理(P<0.05),而革兰氏阴性菌相对丰度显著高于金钗石斛多糖处理(P<0.05)。由此可以看出,金钗石斛多糖改善了隐性白羽鸡的肠道微生态,提高了产蛋后期鸡的生产性能。In order to study the effects of Dendrobium nobile polysaccharides on egg production performance and intestinal flora of recessive white feather chickens in late laying period,white feather chickens were fed with Dendrobium nobile polysaccharides,and the egg production indices of white feather chickens were recorded during feeding.After 60 days,the cecal contents of white feather chickens were collected for 16S rRNA gene sequencing and bioinformatics analysis.The results showed that compared with the control,the total number of eggs,egg production,daily egg production and egg production rate were significantly increased under Dendrobium nobile polysaccharide treatment(P<0.01).In the intestinal microbial community of white feather chickens,the dominant genera were Bacteroides and Rikenellaceae_Rc9_gut group at genus level,and the dominant phyla were Bacteroidota and Fimicutes at phylum level.Compared with the control,the relative abundance of Ruminococcus_torques_group,Faecalicoccus and Merdibacter in Dendrobium nobile polysaccharide treatment increased significantly(P<0.05),and the relative abundance of the pathogenic bacteria such as Barnesiella decreased significantly(P<0.05).The results of phenotypic prediction showed that the relative abundance of Gram positive bacteria in intestinal microbial community of white feather chickens in the control was significantly lower than that in Dendrobium nobile polysaccharide treatment(P<0.05),while the relative abundance of Gram negative bacteria was significantly higher than that in Dendrobium nobile polysaccharide treatment(P<0.05).It can be seed that Dendrobium nobile polysaccharides can improve the intestinal microecology of recessive white feather chickens and improve the production performance of hens in the late laying period.

关 键 词：金钗石斛多糖 产蛋性能 肠道微生物 16S rRNA 隐性白羽鸡 

分 类 号：S831.5[农业科学—畜牧学]