基于SSR标记的MCID法鉴定新疆地方梨品种及其遗传多样性分析  

Identification of local pear cultivars in Xinjiang Uygur Autonomous Region by MCID method based on SSR markers and analysis of their genetic diversity

作　　者：毕清芮 马新院 薛玉冉 樊国全[2] 王邵鹏 牛莹莹 BI Qingrui;MA Xinyuan;XUE Yuran;FAN Guoquan;WANG Shaopeng;NIU Yingying(College of Horticulture,Xinjiang Agricultural University/Research Center of Featured Fruit Trees,Urumqi 830052,China;Luntai Fruit Tree Resource Nursery,Xinjiang Academy of Agricultural Sciences,Luntai 841600,China)

机构地区：[1]新疆农业大学园艺学院/特色果树研究中心,新疆乌鲁木齐830052 [2]新疆农业科学院轮台果树资源圃,新疆轮台841600

出　　处：《江苏农业学报》2024年第10期1933-1941,共9页Jiangsu Journal of Agricultural Sciences

基　　金：新疆维吾尔自治区天池博士计划项目;新疆维吾尔自治区高校基本科研业务费科研项目(XJEDU2022P045)。

摘　　要：为深入探究新疆地方梨品种的遗传关系,本研究采用简单重复序列(SSR)标记技术对28个新疆地方梨品种进行遗传多样性分析。同时,利用人工绘制品种鉴别示意图(MCID)法建立品种鉴定图(CID)。结果表明,新疆地方梨品种间遗传差异较小,遗传分化为中等程度。通过构建新疆地方梨品种的聚类树状图,发现28个新疆地方梨具有较高的遗传多样性,遗传相似性系数为0.52~0.98。群体结构分析结果表明,最佳群体群组数值(K)=3时新疆地方梨品种被划分为3个组,各品种之间存在普遍的基因交流。采用MCID法构建新疆地方梨品种的CID图谱,并验证了该方法的有效性。本研究结果可为今后梨种质资源评价和利用提供理论依据。To further explore the genetic relationship of local pear cultivars in Xinjiang Uygur Autonomous Region,the genetic diversity of 28 local pear cultivars was analyzed by simple sequence repeat(SSR)marker technology.At the same time,the cultivar identification diagram(CID)was established by manual cultivar identification diagram(MCID).The results showed that the genetic differences among the local pear cultivars in Xinjiang Uygur Autonomous Region were small,and the genetic differentiation was moderate.By constructing the clustering tree diagram of local pear cultivars in Xinjiang Uygur Autonomous Region,it was found that 28 local pear cultivars had high genetic diversity,and the genetic similarity coefficient ranged from 0.52 to 0.98.The results of population structure analysis showed that the local pear cultivars in Xinjiang Uygur Autonomous Region were divided into three groups when the best group value(K)was three,and there was a general gene exchange among cultivars.The CID of Xinjiang local pear cultivars was constructed by MCID method,and the effectiveness of the method was verified.The results of this study provide a theoretical basis for the evaluation and utilization of pear germplasm resources in the future.

关 键 词：梨 简单重复序列(SSR) 遗传多样性 品种鉴定 

分 类 号：S661.2[农业科学—果树学]