青花菜多聚半乳糖醛酸酶基因BoPGX3的克隆及盐胁迫下的表达特征分析  

Cloning of Broccoli Polygalacturonase Gene BoPGX3 and Analysis of Its Expression Characteristics under Salt Stress

作　　者：唐征[1] 昂海燕 裴徐梨 杨雅蓉 莫丽玲 荆赞革 TANG Zheng;ANG Haiyan;PEI Xuli;YANG Yarong;MO Liling;JING Zange(College of Agriculture and Biotechnology,Wenzhou Vocational College of Science and Technology,Wenzhou 325006,China;College of Agriculture and Life Sciences,Kunming University,Kunming 650214,China)

机构地区：[1]温州科技职业学院农业与生物技术学院,浙江温州325006 [2]昆明学院农学与生命科学学院,云南昆明650214

出　　处：《蔬菜》2024年第11期29-35,共7页Vegetables

基　　金：云南省地方本科高校(部分)基础研究联合专项(2018FH001-044);云南省科技厅基础研究专项青年项目(2019-1-C-25318000002236);浙江省自然科学基金项目(LY18C150006);浙江省农业(蔬菜新品种选育)新品种选育重大科技专项(2021C02065-4-2)。

摘　　要：多聚半乳糖醛酸酶(PG)可降解细胞壁,从而降低植物抵抗能力。为了解青花菜PG基因响应盐胁迫的分子机制,以青花菜自交系‘WN12-95B’为试验材料,提取青花菜的总RNA并合成cDNA,克隆PG基因,并检测其在盐胁迫下的表达特征。结果表明:青花菜BoPGX3基因序列全长1476 bp,推导编码491个氨基酸,相对分子量为53.44 kD,等电点为5.84。系统进化分析表明,青花菜BoPGX3与羽衣甘蓝、甘蓝、白菜、萝卜和花椰菜的PG基因亲缘关系较近。荧光定量PCR结果显示,BoPGX3基因受盐胁迫诱导表达,处理3 h后相对表达量达到初始表达量的2.9倍,处理12 h时相对表达量下降至初始表达量的1.4倍。综上,青花菜BoPGX3基因参与了盐胁迫的响应,并可能在此过程中发挥着重要的作用。Polygalacturonase(PG)can degrade the cell wall to reduce resistance in plant.In order to understand the molecular mechanism of PG gene response to salt stress in broccoli,the total RNA was extracted and cDNA was synthesized to clone the PG gene of broccoli by taking the inbred line'WN12-95B'as experimental material.Meanwhile the expression characteristics of PG gene were also detected under salt stress.The results showed that the total length of BoPGX3 gene sequenc was 1476 bp in broccoli,deduced encode 491 amino acids,with a relative molecular weight of 53.44 kD and an isoelectric point of 5.84.Phylogenetic analysis showed that BoPGX3 had close genetic relationship with Brassica oleracea var.acephala,Brassica oleracea,Brassica rapa subsp.pekinensis,Raphanus sativus and Brassica oleracea L.var.botrytis.The results of fluorescent quantitative PCR showed that the expression of BoPGX3 gene could be induced with salt stress.After 3 hours of treatment,the relative expression level increased to 2.9 times,and decreased 1.4 times at 12 h after treatment.BoPGX3 can involve in the response to salt stress in broccoli,and may play important roles in this process.

关 键 词：青花菜 多聚半乳糖醛酸酶 基因克隆 盐胁迫 表达特征 亲水性蛋白 氨基酸序列 非生物胁迫 

分 类 号：S635[农业科学—蔬菜学]