金鸡菊苷与CYP3A4/CYP2D6的结合特性和稳定性  

Binding Characteristics and Stability of Coreopsin with CYP3A4/CYP2D6

作　　者：李丽 李媛 陶彦舟 廉笛 崔晶晶 杜雨桐 LI Li;LI Yuan;TAO Yanzhou;LIAN Di;CUI Jingjing;DU Yutong(College of Chemistry,Changchun Normal University,Changchun 130032,China)

机构地区：[1]长春师范大学化学学院,长春130032

出　　处：《吉林大学学报（理学版）》2024年第6期1491-1498,共8页Journal of Jilin University:Science Edition

基　　金：吉林省科技厅国际合作项目(批准号:20210402049GH);长春师范大学研究生创新项目(批准号:YJSCX2024026).

摘　　要：采用光谱分析和计算机模拟技术研究金鸡菊苷与CYP3A4/CYP2D6的结合特性和稳定性.结果表明:金鸡菊苷以静态猝灭为主、动态猝灭为辅的方式猝灭细胞色素P450同工酶(CYPs)的固有荧光;金鸡菊苷与CYP3A4的结合力大于与CYP2D6的结合力;金鸡菊苷与CYPs发生相互作用形成复合物;金鸡菊苷与CYPs结合,导致CYPs二级结构发生改变;金鸡菊苷主要通过氢键和范德华力与CYPs结合;金鸡菊苷与两种CYPs形成的复合物稳定.The binding characteristics and stability of coreopsin with CYP3A4/CYP2D6 was studied by using spectroscopy analysis and computer simulation techniques.The results show that the intrinsic fluorescence of cytochrome P450 proteins(CYPs)is quenched mainly by static quenching and supplemented by dynamic quenching.The binding capacity of coreopsin with CYP3A4 is greater than that of CYP2D6.The coreopsin interacts with CYPs to form a complex.The binding of coreopsin to CYPs leads to changes in the secondary structure of CYPs.The coreopsin mainly binds to CYPs through hydrogen bonds and van der Waals forces.The complex formed by coreopsin and two types of CYPs is stable.

关 键 词：金鸡菊苷 细胞色素P3A4 细胞色素P2D6 结合特性 稳定性 

分 类 号：O65[理学—分析化学]