蛋白质相互作用技术在分子水平的应用  

The Application of Protein Interaction Technology at the Molecular Level

作　　者：韩镌竹 杨文腰 高铎 HAN Juanzhu;YANG Wenyao;GAO Duo(Liaoning Inspection,Examination&Certification,Liaoning Key Laboratory of Livestock Product Safety,Shenyang 110036,China;Liaoning Chengda Biotechnology Co.,Ltd.,Shenyang 110179,China;Shenyang Pharmaceutical University School of Life Sciences and Biopharmaceuticals,Shenyang 110016,China)

机构地区：[1]辽宁省检验检测认证中心[辽宁省农产品及兽药饲料产品检验检测院],辽宁省畜产品安全重点实验室,辽宁沈阳110036 [2]辽宁成大生物股份有限公司,辽宁沈阳110179 [3]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《品牌与标准化》2024年第6期181-183,共3页

摘　　要：基于蛋白复合物在钠离子通道相关疾病中的调控作用,本文综述了蛋白质相互作用构建蛋白质关系的网络和生物学通路中涉及的体内和体外相关检测方法,如酵母双杂交系统、双分子荧光互补、免疫共沉淀技术、融合蛋白Pull-Down技术、单分子下拉、高通量蛋白质芯片、微生物质谱、串联亲和蛋白纯化,以及以生物信息学为主的研究方法,并通过相关检测技术在大分子蛋白复合物中的应用获得新的靶点。Based on the regulatory role of protein complexes in sodium ion channel related diseases,this article reviews the in vivo and in vitro detection methods involved in the construction of protein relationship networks and biological pathways through protein interactions:yeast two hybrid system,bimolecular fluorescence complementarity,immune coprecipitation technology,fusion protein Pull-Down technology,single-molecule pull-down,high-throughput protein chip,microbial mass spectrometry,tandem affinity protein purification,and bioinformatics based research methods.Through the application of relevant detection technologies in large molecule protein complexes,new targets are obtained.

关 键 词：蛋白质-蛋白质相互作用 酵母双杂交系统 双分子荧光互补 免疫共沉淀技术 串联亲和蛋白纯化 

分 类 号：Q503[生物学—生物化学]