福建省Y群脑膜炎奈瑟菌病原学和基因组学分析  

Etiological and genomic analysis of Neisseria meningitidis serogroup Y in Fujian Province

作　　者：高亚东 梁雪晨 郑恩惠[1] 翁顺太[1] 李曲文[1,3] Gao Yadong;Liang Xuechen;Zheng Enhui;Weng Shuntai;Li Quwen(Fujian Provincial Key Laboratory of Zoonosis Research,Fujian Provincial Center for Disease Control and Prevention,Fuzhou 350012,China;School of Public Health,Fujian Medical University,Fuzhou 350108,China;Professional Teaching Practice Base of School of Public Health,Fujian Medical University,Fuzhou 350012,China)

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州350012 [2]福建医科大学公共卫生学院,福州350108 [3]福建医科大学公共卫生学院专业教学实习基地,福州350012

出　　处：《中华微生物学和免疫学杂志》2024年第10期879-885,共7页Chinese Journal of Microbiology and Immunology

基　　金：福建省卫生健康青年科研课题(2022QNA056)。

摘　　要：目的分析引起福建省流行性脑脊髓膜炎(以下简称流脑)病例的Y群脑膜炎奈瑟菌(Neisseria meningitidis,Nm)病原学特征。方法对分离自病例、密切接触者(以下简称密接)和健康人群的8株Nm分离株进行菌株鉴定、血清群鉴定、药物敏感性试验、分子分型、平均核苷酸一致性(average nucleotide identity,ANI)分析、核心基因组多位点序列分型(cgMLST)和单核苷酸多态性(SNP)分析。结果2024年福建省8株分离株均为Y血清群Nm菌株。12种抗菌药物敏感性试验结果表明,2024年福建省分离株均对复方新诺明耐药,NMFJ202406和NMFJ202407分离株对萘啶酸耐药以及对环丙沙星中介,其余对青霉素、头孢噻肟和头孢曲松等抗菌药物均敏感。分子分型结果表明除NMFJ202406分离株的序列型(sequence type,ST)为ST-2039外,其余均为ST-1655,所有分离株的克隆群(clonal complex,CC)均属于CC23,PorA和FetA分型结果显示均为P1.5-1,10-1:F4-1型别。ANI及系统进化分析表明福建省分离株主要分为两簇,分别为NMFJ202401及其密接分离株(NMFJ202402~NMFJ202405),以及NMFJ202406、NMFJ202407和NMFJ202408分离株。相较于广东省、上海市和河北省分离株,福建省分离株分别与加拿大分离株(NML2019-167,2019年)、日本分离株(NIID501,2009年)和英国分离株(CA41956,2015年)亲缘关系更近。结论这是福建省首次发现的Y血清群Nm菌株引起的流脑病例,可能由国外不同来源的菌株传播而来,且已经在多地区发生扩散。今后应加强ACYW135群流脑疫苗的推广使用,以及Y群流脑病例的流行病学和病原学监测。ObjectiveTo analyze the etiological characteristics of epidemic cerebrospinal meningitis caused by serogroup Y Neisseria meningitidis(Nm)in Fujian Province.MethodsThe strain identification,serogroup identification,antimicrobial susceptibility test,molecular typing,average nucleotide identity(ANI),core genome multilocus sequence typing(cgMLST),and single nucleotide polymorphism(SNP)analysis were performed on eight Nm strains isolated from cases of epidemic cerebrospinal meningitis,close contacts,or healthy population.ResultsAll eight isolates from Fujian Province in 2024 were serogroup Y Nm strains.The antimicrobial susceptibility test for 12 antibiotics showed that all isolates from Fujian Province in 2024 were resistant to trimethoprim-sulfamethoxazole,and NMFJ202406 and NMFJ202407 isolates were resistant to nalidixic acid and intermediate to ciprofloxacin,while the others were sensitive to antibiotics such as penicillin,cefotaxime,and ceftriaxone.All isolates were ST-1655 sequence type(ST),except for NMFJ202406 isolate which were ST-2039,and all isolates belonged to clonal complex 23(CC23).The finetyping antigen profile of all isolates were P1.5-1,10-1:F4-1.ANI and phylogenetic analysis indicated that the isolates in Fujian Province were mainly divided into two clusters,namely NMFJ202401 and its close contact isolates(NMFJ202402-NMFJ202405),as well as NMFJ202406,NMFJ202407,and NMFJ202408 isolates.Compared to the isolates from Guangdong,Shanghai,and Hebei Provinces,the strains isolated in Fujian Province were more closely related to the strains isolated in Canada(NML2019-167,2019),Japan(NIID501,2009),and the United Kingdom(CA41956,2015),respectively.ConclusionsThis is the first report of epidemic cerebrospinal meningitis caused by serogroup Y Nm isolates in Fujian Province,which might be transmitted from different sources abroad and have spread in many areas.In the future,the wider usage of tetravalent meningococcal vaccine ACYW135 is needed,and the epidemiological and etiological surveillance of serogroup Y epi

关 键 词：流行性脑脊髓膜炎 脑膜炎奈瑟菌 Y血清群 CC23克隆群 

分 类 号：R515.2[医药卫生—内科学] R446.5[医药卫生—临床医学]