S100A10对巨噬细胞介导的炎症及迁移的影响  

Effect of S100 A 10 macrophage-mediated inflammation and migration

作　　者：李骄阳 刘晟男 赵雨欣 高静涛 王辉 LI Jiaoyang;LIU Shengnan;ZHAO Yuxin;GAO Jingtao;WANG Hui(College of Medical Laboratory Medi-cine,Xinxiang Medical University,Xinxiang 453003,China;Department of Immunology,School of Basic Medi-cine,Xinjiang Medical University,Urumqi 830054,China;Henan Key Laboratory of Immunology and Targeted Drugs,Collaborative Innovation Center of Molecular Diagnosis and Medical Testing Technology of Henan Province,Xinxiang 453003,China)

机构地区：[1]新乡医学院医学检验学院,新乡453003 [2]新疆医科大学基础医学院免疫学教研室,乌鲁木齐830054 [3]河南省免疫与靶向药物重点实验室,河南省分子诊断与医学检验技术协同创新中心,新乡453003

出　　处：《中国免疫学杂志》2024年第11期2257-2261,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(U21A20366);国家111计划项目(D20036)。

摘　　要：目的:探讨S100A10对巨噬细胞介导的炎症及迁移的影响。方法:建立S100A10-KO的C57BL/6J小鼠模型和S100A10-KO的RAW264.7细胞系。取小鼠腹腔巨噬细胞(PMs)和骨髓巨噬细胞(BMDMs),复苏RAW264.7细胞系并收集细胞。采用qRT-PCR检测S100A10敲除对巨噬细胞炎症因子分泌情况的影响;划痕实验和Transwell检测S100A10敲除对巨噬细胞迁移的影响;CCK8试剂盒检测S100A10敲除对RAW264.7细胞增殖的影响;qRT-PCR和Western blot检测基质金属蛋白酶9(MMP9)、非肌性肌球蛋白重链9(MYH9)的表达。结果:S100A10敲除后,RAW264.7、PMs、BMDMs分泌的炎症因子IL-6、IL-1β、MCP-1水平降低(P<0.05);细胞划痕和Transwell表明S100A10敲除抑制巨噬细胞的迁移;CCK8实验表明S100A10敲除后巨噬细胞的增殖能力减弱;qRT-PCR和Western blot实验表明迁移相关蛋白MMP9、MYH9在S100A10敲除后降低。结论:S100A10敲除后巨噬细胞分泌的炎症因子减少且巨噬细胞迁移和增殖的能力减弱。Objective:To investigate the effect of S100A10 macrophage-mediated inflammation and migration.Methods:C57BL/6J mouse model of S100A10-KO,and RAW264.7 cell line of S100A10-KO were established.Taking mice's peritoneal macro-phages(PMs)and bone marrow macrophages(BMDMs),resuscitating RAW264.7 cell lines,and collecting cells.qRT-PCR was used to detect the effect of S100A10 knockout on the secretion of inflammatory factors in macrophages;wound healing assay and Transwell assay were used to detect the effect of S100A10 knockout on macrophage migration;CCK8 kit was used to detect the effect of S100A10 knockout on the proliferation of RAW264.7 cells;qRT-PCR and Western blot were used to detect the expressions of matrix metallopro-teinase 9(MMP9)and non-muscle myosin heavy chain 9(MYH9).Results:After S100A10 knockout,the inflammatory factors IL-6,IL-1βand MCP-1 levels(P<0.05)secreted by RAW264.7,PMs and BMDMs were excreted decreased;cell scratches and Transwell showed that S100A10 knockout inhibited macrophage migration;CCK8 experiments showed that the proliferation capacity of macro-phages weakened after S100A10 knockout;qRT-PCR and Western blot experiments showed that the migration-related proteins MMP9 and MYH9 decreased after S100A10 knockout.Conclusion:S100A10 knockout decreases the secretion of inflammatory factors by macrophages and attenuated the migration and proliferation of macrophages.

关 键 词：巨噬细胞 S100A10 炎症因子 迁移 

分 类 号：R399[医药卫生—基础医学]