急性ST段抬高型心肌梗死早期外周血Tc17细胞表面标志和细胞因子表达  

Surface markers and cytokine expression of blood Tc 17 cells in early stage of acute ST-elevation myocardial infarction

作　　者：刘畅[1] 孙丽琴[1] 舒龙 张波[1] LIU Chang;SUN Liqin;SHU Long;ZHANG Bo(Affiliated Renhe Hospital of China Three Gorges University,Yichang 443000,China)

机构地区：[1]三峡大学附属仁和医院,宜昌443000

出　　处：《中国免疫学杂志》2024年第11期2350-2354,2360,共6页Chinese Journal of Immunology

基　　金：湖北省卫生健康委员会联合基金项目(WJ2019H545)。

摘　　要：目的:检测急性ST段抬高型心肌梗死(STEMI)早期外周血中表达IL-17A的CD8^(+)T细胞,即Tc17细胞的表型和功能。方法:收集健康人和STEMI患者外周血白细胞,流式细胞术检测CCR6、CD161、CCR5、IL-23R、RORγt表达确定Tc17细胞。流式细胞术检测Tc17细胞活化状态、细胞因子表达和增殖状况。结果:健康志愿者和STEMI患者CCR6^(+)CD161^(+)CD8^(+)T细胞均高表达CCR5、IL-23R和RORγt,被鉴定为Tc17细胞。与健康志愿者相比,STEMI患者Tc17细胞在总CD8^(+)T细胞中的比例升高,且表型上更偏向于效应型记忆性T细胞。健康志愿者Tc17细胞主要表达IL-17A和IL-22,而STEMI患者Tc17细胞不仅表达IL-17A和IL-22,也表达IL-10和TGF-β。但健康志愿者和STEMI患者Tc17细胞均不表达IFN-γ、IL-5、Foxp3和Ki67。结论:STEMI早期Tc17细胞表达IL-17A和IL-22同时上调IL-10和TGF-β表达,可能参与和调节STEMI后急性炎症反应。Objective:To investigate phenotype and function of IL-17A-expressing CD8^(+)T cells(i.e.Tc17 cells)in early stage of acute ST-elevation myocardial infarction(STEMI).Methods:Blood leukocytes were harvested from healthy donors and STEMI patients,and Tc17 cells were recognized by flow cytometry based on expressions of CCR6,CD161,CCR5,IL-23R and RORγt.Activation status,cytokine expression profiles and proliferation of Tc17 cells were evaluated by flow cytometry.Results:CCR6^(+)CD161^(+)CD8^(+)T cells,either in healthy donors or STEMI patients,highly expressed CCR5,IL-23R and RORγt.Therefore,they were identified as Tc17 cells.Compared with healthy donors,frequency of Tc17 cells in total CD8^(+)T cells was increased in STEMI patients.Besides,phenotype of Tc17 cells in STEMI patients was more skewed towards effector memory T cells.Tc17 cells in healthy donors predominantly expressed IL-17A and IL-22,whereas Tc17 cells in STEMI patients expressed IL-10 and TGF-βin addition to IL-17A and IL-22.However,Tc17 cells,either in healthy donors or STEMI patients,did not express IFN-γ,IL-5,Foxp3,and Ki67.Conclu-sion:In early stage of STEMI,Tc17 cells up-regulate IL-10 and TGF-βexpressions while producing IL-17A and IL-22,probably participate in and regulate post-STEMI acute inflammation.

关 键 词：心肌梗死 CD8^(+)T细胞 Tc17细胞 炎症 细胞因子 

分 类 号：R540.41[医药卫生—心血管疾病]