人类免疫缺陷病毒储存库检测方法的现状及应用前景  

Current situation and application prospect of human immunodeficiency virus reservoir detection methods

作　　者：赵豪梅 张清燕 桑锋[2,3] 杨瑶瑶 邓博文 袁硕阳[1] 李强 ZHAO Haomei;ZHANG Qingyan;SANG Feng;YANG Yaoyao;DENG Bowen;YUAN Shuoyang;LI Qiang(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450000,China;The First Affiliated Hospital of the Henan University of Traditional Chinese Medicine,Zhengzhou 450000,China;Henan Key Laboratory of Viral Diseases Prevention and Treatment of Traditional Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]河南中医药大学药学院,郑州450000 [2]河南中医药大学第一附属医院,郑州450000 [3]河南省病毒性疾病中医药防治重点实验室,郑州450000

出　　处：《中国免疫学杂志》2024年第11期2444-2451,共8页Chinese Journal of Immunology

基　　金：河南省重点研发与推广专项科技攻关项目(222102310074);河南省卫生健康委国家中医临床研究基地科研专项(2021JDZY080,2022JDZX019);河南省中医药科学研究专项(20-21ZY2101);河南省特色学科中医学学科建设项目(STG-ZYXKY-2020029)。

摘　　要：获得性免疫缺陷综合征是由人类免疫缺陷病毒(HIV)引起的高死亡率传染病。即使经过抗逆转录病毒治疗,原病毒整合在宿主基因组形成病毒储存库仍可逃避宿主免疫监视及清除。现有的HIV储存库检测方法主要包括细胞体外培养诱导病毒生长试验和基于PCR技术直接检测原病毒基因组方法。了解并精准检测HIV储存库可为治疗HIV提供可靠的技术依据。本文通过对近年来HIV储存库的各种检测方法及其优缺点进行总结分析,以期能够选择出针对不同检测对象的HIV储存库的最优检测方法,并给出恰当建议,为艾滋病的进一步深入研究提供理论指导与技术支撑。Acquired immune deficiency syndrome is an infectious disease with high mortality caused by human immunodefi-ciency virus(HIV).Even after antiretroviral therapy,the virus reservoir formed by the provirus integrated in the host genome can evade host immune surveillance and clearance.The existing methods of HIV reservoir detection mainly include cell culture induced virus growth assay in vitro and direct detection of the provirus genome based on PCR technology.Understanding and measuring accurately of HIV reservoir can provide reliable technical basis for HIV treatment research.This article summarizes and analyzes various detection methods of HIV reservoirs in recent years and their advantages and disadvantages,in order to select the suitable method for different detection objects of HIV reservior,and give appropriate suggestions,to provide technical support and theoretical guidance for further research on acquired immune deficiency syndrome.

关 键 词：人类免疫缺陷病毒 储存库 病毒生长试验 PCR 检测方法 

分 类 号：R512.91[医药卫生—内科学]