基于UHPLC-MS/MS的红肉猕猴桃中花色素检测方法研究  

Development of a UHPLC-MS/MS Method for the Detection of Anthocyanins in Red Flesh Kiwifruit

作　　者：董莹 陈子凡 王国胜 池少铃 谢珊珊 郑少锋 张南峰 DONG Ying;CHEN Zi-Fan;WANG Guo-Sheng;CHI Shao-Ling;XIE Shan-Shan;ZHENG Shao-Feng;ZHANG Nan-Feng(Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection,Guangzhou 510700;Technology Center of Huangpu Customs,Dongguan 523000;Heyuan Food Inspection Institute,Heyuan 517000;Technology Center of Heyuan Customs,Heyuan 517000;Heyuan Drug Inspection Institute,Heyuan 517000;Guangzhou Caizhilin Pharmaceutical Co.,Ltd.,Guangzhou 510000)

机构地区：[1]广东省口岸安全智能化检测重点实验室,广州510700 [2]黄埔海关技术中心,东莞523000 [3]河源市食品检验所,河源517000 [4]河源海关技术中心,河源517000 [5]河源市药品检验所,河源517000 [6]广州采芝林药业有限公司,广州510000

出　　处：《中国口岸科学技术》2024年第10期65-71,共7页China Port Science and Technology

基　　金：广东省口岸安全智能化检测重点实验室项目(2023B1212010011);河源市社会发展科技计划项目(230613111602379)。

摘　　要：本文建立了一种基于超高效液相色谱-质谱联用法(Ultra High-Performance Liquid Chromatography-Mass Spectrometry,UHPLC-MS/MS)测定红肉猕猴桃中矢车菊素、飞燕草素、矮牵牛素、花葵素、芍药素、锦葵色素的方法。样品经含0.1%甲酸的甲醇水溶液直接提取,在电喷雾电离源(Electron Spray Ionization,ESI)于正离子模式下,采用选择性反应监测(Selected Reaction Monitoring,SRM)进行检测。结果显示,在5~500 ng/mL含量之间质量浓度范围内具有良好线性关系,线性相关系数r均在0.997以上,回收率为65.0%~99.6%,精密度为4.1%~9.1%,检出限为0.0003 mg/kg,定量限为0.001 mg/kg。结果表明,该方法可对红肉猕猴桃中6种花色素物质进行含量检测,具有简便高效、结果准确、灵敏度高、重复性好的特点。This study established a method for the determination of cyanidin,delphinidin,petunidin,malvidin,peonidin,and pelargonidin in red flesh kiwifruit using ultra high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Samples were directly extracted with a methanol aqueous solution containing 0.1%formic acid and analyzed using electrospray ionization(ESI)in positive ion mode with selected reaction monitoring(SRM).The results showed good linearity within the concentration range of 5-500 ng/mL,with linear correlation coefficients(r)above 0.997.The recovery rates were between 65.0%and 99.6%,precision ranged from 4.1%to 9.1%,and the limits of detection and quantification were 0.0003 mg/kg and 0.001 mg/kg,respectively.The method demonstrated its capability for the content determination of six anthocyanin compounds in red flesh kiwifruit,characterized by simplicity,efficiency,accuracy,high sensitivity,and good repeatability.

关 键 词：红肉猕猴桃 花色素 超高效液相色谱-质谱 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]