西安市2019—2023年分离的乙型Victoria系流感病毒血凝素和神经氨酸酶基因特征分析  

Analysis of hemagglutinin and neuraminidase gene characteristics of influenza B(Victoria)viruses isolated in Xi′an city from 2019 to 2023

作　　者：邓杨妮 张鹏 杨玉捷 韩月雯 段媛媛 李淑玲 黄银娟[1] 魏嘉宁 张晔[1] 吴瑞[1] 陈海龙[1] Deng Yangni;Zhang Peng;Yang Yujie;Han Yuewen;Duan Yuanyuan;Li Shuling;Huang Yinjuan;Wei Jianing;Zhang Ye;Wu Rui;Chen Hailong(Xi′an Center for Disease Control and Prevention,Xi′an 710054,China)

机构地区：[1]西安市疾病预防控制中心,西安710054

出　　处：《中华实验和临床病毒学杂志》2024年第5期552-557,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：西安市科技计划项目(22YXYJ0062,23YXYJ0092)。

摘　　要：目的了解西安市2019—2023年分离的乙型Victoria系流感病毒(BV)血凝素(hemagglutinin,HA)和神经氨酸酶(neuraminidase,NA)的基因特性和遗传进化特征。方法收集西安市流感监测网络实验室2019—2023年分离的BV毒株25株,利用MiniSeq高通量测序平台对HA和NA基因进行测序;利用生物信息学软件构建进化树,分析同源性及变异位点,在线预测N-糖基化位点;通过血凝抑制试验分析毒株的抗原性。结果西安市2019—2023年BV型流感呈季节性传播,流行高峰集中在冬春季。HA基因进化分析结果显示,西安市2019年毒株属于V1A.3分支,2021—2023年毒株属于V1A.3a.2分支。抗原位点分析显示,2021—2022年BV毒株HA蛋白与2019年相比有3个抗原决定簇的6个位点发生变异,2023年HA蛋白与2021—2022年相比变化了1个抗原决定簇的2个位点。2019年西安市BV毒株NA基因进化上属于A.1.1分支,到2021和2022年进化为A.1.2分支,2023年又进化为B分支及其衍生株,所有毒株均未发生NA抑制剂相关耐药位点变异。抗原分析结果显示西安市BV毒株多为疫苗组分株的类似株。糖基化分析结果显示2021—2023年毒株HA蛋白潜在糖基化位点比2019年毒株少1个,而NA蛋白的潜在糖基化位点仅有2023年个别毒株发生改变。结论2019—2023年西安市BV毒株HA和NA基因在不断发生变异,进化成新的分支。自2021年起V1A.3a.2成为HA基因优势进化分支,而2019—2023年NA基因进化分支不断改变。ObjectiveTo investigate the genetic and evolutionary characteristics of hemagglutinin(HA)and neuraminidase(NA)genes of influenza B/Victoria(BV)virus in Xi′an city from 2019 to 2023.MethodsTwenty-five BV strains isolated from the Xi′an influenza surveillance network laboratory between 2019 and 2023 were collected.The HA and NA genes were sequenced using MiniSeq high-throughput sequencing platform.An evolutionary tree was constructed using bioinformatics software to analyze homology and mutation sites,and to predict N-glycosylation sites online.The antigenicity of the strains was analyzed through hemagglutination inhibition tests.ResultsThe BV influenza in Xi′an exhibited a distinct seasonal transmission pattern from 2019 to 2023,with peak prevalence occurring during the winter and spring seasons.The evolutionary analysis of the HA genes shows that the strains from Xi′an in 2019 belong to the V1A.3 branch,and the strains from 2021 to 2023 belong to the V1A.3a.2 branch.Analysis of antigenic sites showed that there were variations in 6 sites of 3 antigenic determinants in the HA proteins of the BV strains from 2021-2022 compared to 2019,and 2 sites of 1 antigenic determinant changed in the HA proteins in 2023 compared to 2021-2022.The evolutionary analysis of the NA genes indicates that the BV strains from Xi′an in 2019 belong to the A.1.1 branch.By 2021 and 2022,it had evolved into the A.1.2 clade,and by 2023,it had further evolved into the B clade and its derivatives,with no strains showing mutations associated with resistance to NA inhibitors.Antigenic analysis indicated that the majority of BV strains in Xi′an were similar to the strains included in the vaccine composition.Furthermore,glycosylation analysis showed that the potential N-glycosylation sites in the HA proteins of BV strains from 2021-2023 were reduced by one compared to those from 2019,and only a few strains from 2023 displayed alterations in the potential N-glycosylation sites of the NA proteins.ConclusionsThe HA and NA genes of the BV s

关 键 词：乙型流感病毒 血凝素 神经氨酸酶 遗传进化分析 

分 类 号：R373.13[医药卫生—病原生物学]