木质素单体跨膜转运的技术研究进展  

Advances in Technology for Transport of Monolignols across Membrane

作　　者：毛馨艺 兰芸 张准 张叶卓 金琪[1,2,3,4] 赵美棋 曾子柽 李晔 MAO Xin-yi;LAN Yun;ZHANG Zhun;ZHANG Ye-zhuo;JIN Qi;ZHAO Mei-qi;ZENG Zi-cheng;LI Ye(State Key Laboratory of Tree Genetics and Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;National Engineering Laboratory for Tree Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083)

机构地区：[1]北京林业大学生物科学与技术学院林木遗传育种全国重点实验室,北京100083 [2]北京林业大学生物科学与技术学院林木育种与生态修复国家工程研究中心,北京100083 [3]北京林业大学生物科学与技术学院林木、花卉遗传育种教育部重点实验室,北京100083 [4]北京林业大学生物科学与技术学院树木花卉育种生物工程国家林业和草原局重点实验室,北京100083

出　　处：《生物技术通报》2024年第10期198-207,共10页Biotechnology Bulletin

基　　金：北京林业大学“5·5工程”科研创新团队项目(BLRC2023A01);北京林业大学大学生创新创业训练计划(X202310022101);国家重点研发计划(2022YFF0712500)。

摘　　要：木质素(lignin)作为一种重要的可再生资源,主要集中在植物的次生细胞壁中,在植物的营养物质运输、机械支持和病原体防御等生理过程中发挥着关键作用。木质素的形成过程包括3个主要环节:单体的胞内合成、跨膜转运以及胞外聚合。深入探究木质素单体跨膜转运的分子机制,对揭示细胞壁形成分子机制及木材改良具有重要意义。基于此,本综述从转录组学、基因工程、点击化学、荧光显微成像、分子模拟等技术角度,对木质素单体跨膜转运的相关进展和最新技术进行了梳理和总结,阐述了木质素单体跨膜转运相关研究的新技术和存在的技术瓶颈,并对比分析了不同技术之间的优缺点,最后提出了木质素单体跨膜转运机制研究中仍存在的问题和面临的挑战。相信随着成像技术、结构生物学、人工智能等科技的飞速发展,将为进一步阐明木质素单体跨膜转运的分子机理提供有效的技术手段。期望本综述为木质素单体的相关研究提供新的思路和方法。As an important renewable resource,lignin is mainly deposited in the secondary cell wall of vascular plants and plays an important role in physiological processes such as nutrient transport,mechanical support,and plant pathogen defense.The formation of lignin can be divided into three processes:Intracellular synthesis,transmembrane transport and extracellular polymerization of monolignols.Exploring the transmembrane transport mechanism of monolignols is of great significance for revealing the mechanism of cell wall formation and wood improvement..In this review,we sorted and summarized the relevant progress and latest technologies of monolignols transmembrane transport from the perspectives of transcriptomics,genetic engineering,click chemistry,fluorescence microscopy,and molecular simulation.Then we described the new technologies and existing technical bottlenecks in the research on monolignol transmembrane transport.In addition,we compared and analyzed the advantages and disadvantages of different technologies.Finally,we proposed the issues and challenges in the study of the mechanism of monolignols transmembrane transport.It is believed that the rapid development of imaging technology,structural biology,artificial intelligence and other technologies will provide new tools for further investigating the transmembrane transport mechanism of monolignols.This review is expected to provide new methods for the study of monolignols.

关 键 词：木质素单体 跨膜转运 点击化学 分子模拟 荧光显微成像 

分 类 号：Q945[生物学—植物学]