组学技术在生防芽胞杆菌的应用进展  

Progress in the Application of Omics Technology in Biocontrol Bacillus

作　　者：许沛冬 易剑锋 陈迪 陈浩 谢丙炎 赵文军 XU Pei-dong;YI Jian-feng;CHEN Di;CHEN Hao;XIE Bing-yan;ZHAO Wen-jun(Center for Biosafety,Chinese Academy of Inspection and Quarantine,Sanya 572024;Chinese Academy of Inspection and Quarantine,Beijing 100000;School of Tropical Agriculture and Forestry,Hainan University,Haikou 570228)

机构地区：[1]三亚中国检科院生物安全中心,三亚572024 [2]中国检验检疫科学研究院,北京100000 [3]海南大学热带农林学院,海口570228

出　　处：《生物技术通报》2024年第10期208-220,共13页Biotechnology Bulletin

基　　金：三亚崖州湾科技城“崖州湾”菁英人才科技专项项目(SCKJ-JYRC-2023-44);海南省自然科学基金青年项目(324QN365);海南省“南海新星”科技创新人才平台项目(NHXXRCXM202358);三亚市科技创新专项项目(2022KJCX99)。

摘　　要：芽胞杆菌(Bacillus spp.)是全世界应用最广泛的生防微生物菌种资源之一,在基因组学成为普遍性基础学科的时代,转录组、蛋白质组、代谢组等多组学技术的联用成为深入揭示芽胞杆菌生物学特性和生防机制研究的重要手段。目前NCBI数据库共计发布了10813个芽胞杆菌全基因组序列,其中装配注释完整的共1842个,占总数量的17.04%。对芽胞杆菌基因组与比较基因组的分析发现,次级代谢产物基因簇是芽胞杆菌基因组中最具有保守性,又具有特异性的部分,它们既是天然产物的重要来源,也在细菌间以及细菌与环境的相互作用和生命周期中扮演着重要角色。截至目前,对于芽胞杆菌的相关研究,多采取整合组学模式和生物信息学计算方法,聚焦于芽胞杆菌的抗性形成、生物膜形成、定殖、促生、诱导植物抗逆性等机制。随着研究的不断深入,芽胞杆菌生防过程中的基因调控机制、蛋白质表达和代谢途径将进一步解析,这将有助于发现新的生防活性物质和优化生防策略。本文综述了转录组、蛋白质组、代谢组等多组学技术在芽胞杆菌上的研究进展,以期为芽胞杆菌生防作用机制的解析以及生防菌株改良的深入研究提供参考。Bacillus is one of the most widely used biocontrol microbial resources in the world.In the era when genomics has become a universal basic discipline,the combined application of transcriptomics,proteomics,metabonomics and other multi-omics technologies have become an important means to deeply reveal the biological characteristics and biocontrol mechanisms of Bacillus.Currently,the NCBI database has published a total of 10813 genome sequences of Bacillus,of which 1842 have complete assembly annotations,accounting for 17.04%of the total number.The secondary metabolite gene clusters are the most conserved and specific part of the Bacillus genome.They are not only an important source of natural products,but also play important roles in the interactions and life cycle between bacteria and the environment.Up to now,research on Bacillus has mostly adopted integrated omics models and bioinformatics calculation methods,focusing on the mechanisms of Bacillus resistance formation,biofilm formation,colonization,growth promotion,and induction of plant stress resistance.With the continuous deepening of research,the gene regulatory mechanisms,protein expression,and metabolic pathways in the biocontrol process of Bacillus will be further elucidated,which will help discover new biocontrol active substances and optimize biocontrol strategies.This article reviews the research progress of multi-omics techniques such as genomics,transcriptomics,proteomics,and metabonomics on Bacillus.It aims to provide reference for the analysis of the biocontrol mechanism of Bacillus and the in-depth study of biocontrol strain improvement.

关 键 词：芽胞杆菌 基因组学 转录组学 蛋白质组学 代谢组学 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q78[农业科学—植物保护]