禾本科植物内生真菌研究22:Epichloe festucae E2368基因组中非核糖体肽合成酶编码基因的挖掘与分析  

Grass endophyte researches 22:genomic analysis of non-ribosomal peptide synthetases genes in Epichloe festucae E2368

作　　者：纪燕玲[1] 韩魁 茅冬梅[1] 陈永敢 王志伟[1] JI Yanling;HAN Kui;MAO Dongmei;CHEN Yonggan;WANG Zhiwei(College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China;College of Fisheries and Life Science,Hainan Tropical Ocean University,Sanya 572022,China)

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]海南热带海洋学院水产与生命学院,海南三亚572022

出　　处：《南京农业大学学报》2024年第6期1097-1104,共8页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30800156,30970081,31372365)。

摘　　要：[目的]Epichloe内生真菌能够产生抵御牲畜和害虫啃食的生物碱,其中部分生物碱由非核糖体肽合成酶(non-ribosomal peptide synthetase,NRPS)合成。本研究旨在分析E.festucae基因组内的NRPS编码基因,并预测其功能。[方法]以E.festucae E2368菌株基因组数据为对象,综合运用HMMER和全局比对等分析方法以及ClustScan、PKS/NRPS Analysis和NCBI等数据库进行信息比对。[结果]E2368基因组中含有至少19个候选NRPS基因,其中11个未在Epichloe内生真菌中报道。序列比对结果表明候选基因均为子囊菌中已报道的序列,但其中8个基因的功能未知;E2368基因组中存在环孢菌素合成酶基因扩增现象。构建候选NRPS基因所有A结构域氨基酸序列发育树,发现不同NRPS基因的A结构域会聚到同一分枝,而相同NRPS基因的A结构域则分散于不同分枝,说明采用简并引物扩增NRPS基因存在一定的局限性。[结论]对E2368基因组中NRPS基因的挖掘,将有助于解析NRPS基因的多样性,促进对新的天然产物的发现和生物合成途径的认识。[Objectives]Epichloe endophytes confer a series alkaloids of biotic resistance from insect feeding deterrents to herbivory alkaloid toxins synthesized by non-ribosomal peptide synthetases(NRPS).This study aimed to investigate the NRPS genes in the fungal genome and predict their functions.[Methods]The genome sequences of E.festucae E2368 was analyzed by HMMER and global comparison,ClustScan,PKS/NRPS Analysis,and NCBI database.[Results]Nineteen candidate NRPS genes and 11 of them had not been described in Epichloe spp..All the genes were reported sequences in ascomycetes,but the functions of 8 genes had not been determined after sequence alignment.Cyclosporine synthesis genes might have three copies in the E2368 genome.By building the maximum parsimony tree by all A domain sequences of the 19 candidate genes,the A domains from different NRPS genes were listed together,while the A domains of the same NRPS genes were separated into different branches.The limitations of degenerated primers might exist.[Conclusions]NRPS genes within E2368 genome will reveal the diversity of the NRPS genes,as well as the discovery and biological synthesis pathway of new natural products.

关 键 词：NRPS数据库 基因挖掘 生物碱 类似度分析 禾本科植物内生真菌 

分 类 号：Q933[生物学—微生物学]