基于磷脂酰肌醇3-激酶/蛋白激酶B信号通路探讨麦粒灸对肺炎链球菌肺炎大鼠的治疗作用  

Therapeutic effect of wheat grain moxibustion on Streptococcus pneumoniae pneumonia in rats based on the PI3K/Akt signaling pathway

作　　者：曹金艳 马晶鑫 董思琪 肖扬 关伟 李元宾 CAO Jinyan;MA Jingxin;DONG Siqi;XIAO Yang;GUAN Wei;LI Yuanbin(Shanxi University of Chinese Medicine,Taiyuan,Shanxi 030000;Endocrine and Metabolic Center,Taiyuan Central Hospital,Taiyuan,Shanxi 030000;Department of Endocrinology,the Third People's Hospital of Longgang District,Shenzhen,Guangdong 518000)

机构地区：[1]山西中医药大学,山西太原030000 [2]山西省太原市中心医院内分泌代谢中心,山西太原030000 [3]广东省深圳市龙岗区第三人民医院内分泌科,广东深圳518000

出　　处：《河北中医》2024年第11期1833-1837,1842,共6页Hebei Journal of Traditional Chinese Medicine

基　　金：山西省教育厅高等学校科技创新计划项目(晋教科函[2021]20号);山西省卫生健康委科研课题(编号:2022112929)。

摘　　要：目的观察麦粒灸对肺炎链球菌肺炎大鼠磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路的影响,探讨麦粒灸治疗肺炎链球菌肺炎的作用机制。方法将30只SD大鼠随机分为模型组、对照组和治疗组,每组10只。模型组和治疗组经滴鼻法滴入50μL肺炎链球菌混悬液,对照组滴入等容积的0.9%氯化钠注射液,各组均操作1次,模型建成后治疗组采用麦粒灸进行干预,干预7 d。期间监测大鼠的生存状态,14 d后处死大鼠,检测大鼠肺脏组织的炎症水平、肺泡灌洗液中的细胞因子水平[白细胞介素6(IL-6)、IL-8、肿瘤坏死因子α(TNF-α)]和PI3K/Akt通路蛋白的含量和活性。结果与对照组比较,模型组的生存状态下降,肺脏组织炎症反应明显,肺泡灌洗液中IL-6、IL-8、TNF-α等炎症因子水平上升。治疗组经麦粒灸治疗后,肺脏组织中PI3K/Akt信号通路活性降低(P<0.05),肺泡灌洗液中IL-6、IL-8、TNF-α等炎症因子水平下降。结论麦粒灸可能抑制PI3K/Akt通路的活性,降低炎症因子表达,减轻炎症反应,从而起到治疗肺炎链球菌肺炎的作用。Objective To explore the influence of wheat grain moxibustion on Streptococcus pneumoniae pneumonia and the phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)signaling pathway in rats,and the underlying mechanism.Methods A total of 30 Sprague-Dawley(SD)rats were randomly divided into model group,control group and treatment group,with 10 rats per group.Rats in the model group and the treatment group were given 50μL nasal drop of Streptococcus pneumoniae suspension,and those in the control group were similarly given the same amount of normal saline once.Wheat grain moxibustion for 7-day intervention was performed in the treatment group.Survival was monitored,and rats were sacrificed at 14 days.Inflammatory levels in rat lung tissue,cytokine levels(interleukin 6[IL-6],IL-8 and tumor necrosis factor alpha[TNF-α])in alveolar lavage fluid,and expression levels of key proteins in the PI3K/Akt signaling pathway were detected.Results In comparison to the control group,rats in the model group presented survival statue decline,obvious inflammation in the lungs,and higher levels of inflammatory factors(IL-6,IL-8 and TNF-α).After the intervention,the treatment group presented significantly downregulated the expression levels of key proteins in the PI3K/Akt signaling pathway in rat lung tissue,and decreased inflammatory levels in the lung(P<0.05).Conclusion Wheat grain moxibustion significantly decreases relative levels of inflammatory factors,alleviate inflammatory response and treats Streptococcus pneumoniae pneumonia by inhibiting the PI3K/Akt signaling pathway.

关 键 词：肺炎链球菌肺炎 大鼠 动物模型 PI3K/AKT信号通路 麦粒灸 动物实验 

分 类 号：R563.1[医药卫生—呼吸系统] R245.8[医药卫生—内科学]