基于DNA特征序列标记的南沙参基原鉴定研究  

Identification of Original Plants of Adenophorae Radix Based on DNA Signature Sequence

作　　者：王多梅 蒲婧哲 胡冲 陈灵丽 蒋超[4] 袁媛[5] 张亚中 WANG Duo-mei;PU Jing-zhe;HU Chong;CHEN Ling-li;JIANG Chao;YUAN Yuan;ZHANG Ya-zhong(Drug Inspection and Research Institute,Anhui Institute of Food and Drug Control,Hefei 230051,China;NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine,Hefei 230051,China;Huainan Food and Drug Inspection Center,Huainan 232000,China;National Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Medical Experimental Center of China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]安徽省食品药品检验研究院药品检验研究所,安徽合肥230051 [2]国家药品监督管理局中药质量研究与评价重点实验室,安徽合肥230051 [3]淮南市食品药品检验中心,安徽淮南232000 [4]中国中医科学院中药资源中心道地药材品质保障与资源持续利用全国重点实验室,北京100700 [5]中国中医科学院医学实验中心,北京100700

出　　处：《中国现代中药》2024年第11期1848-1853,共6页Modern Chinese Medicine

基　　金：安徽省药品监督管理局监管科学研究重点项目(AHYJ-KJ-202308);安徽省药品监督管理局科技创新项目(AHYJ-KJ-202313)。

摘　　要：目的:建立快速、便捷、准确的南沙参及其混伪品特异性DNA特征序列(DSS)标记分子鉴定方法。方法:采用植物叶绿体基因组数据库(CGIR)获取叶绿体基因组序列,采用IdenDSS软件获得待测物种DSS组及其特异性引物对,通过DNA提取、聚合酶链式反应扩增、测序、序列比对验证,检测待测样品的基因组中是否含有目标物种的DSS或其反向互补序列,进行基原鉴定。结果:通过对筛选的5对DSS与南沙参及其混伪品序列的比对验证,最终确定1组可用于南沙参基原鉴定的DSS标记引物,并将该序列上传至中药分子鉴定平台标准数据库中,结合数据库的BLAST功能,可实现南沙参基原的快速、准确鉴定。结论:筛选的DSS标记特异性强、准确度高,可用于南沙参的基原鉴定。Objective:To develop a rapid,simple,and accurate molecular identification method based on DNA signature sequence(DSS)for distinguishing Adenophorae Radix from its adulterants.Methods:The Chloroplast Genome Information Resource(CGIR)was used to obtain chloroplast genome sequences,and IdenDSS was employed to screen for DSS and specific primers of the original plants of Adenophorae Radix.DNA extraction,PCR amplification,sequencing,and sequence alignment were then carried out to examine whether the genomic DNA of the sample to be detected contained DSS or the reverse complementary sequence of the target species.Results:After comparison of the selected 5 DSSs for Adenophorae Radix and its adulterants,the DSS and primers that can be used for the identification of Adenophorae Radix were finally determined.The DSS was uploaded to the Traditional Chinese Medicine Molecular Identification Platform.The combination of DSS with BLAST achieved rapid and accurate identification of the original plant of Adenophorae Radix.Conclusion:The DSS selected had strong specificity and high accuracy and could be effectively applied in the identification of the original plant of Adenophorae Radix.

关 键 词：南沙参 DNA特征序列 基原鉴定 

分 类 号：R282.5[医药卫生—中药学]