不同国家(地区)穿心莲的质量评价  

Quality Evaluation of Andrographis paniculata from Different Countries and Regions

作　　者：闫炳雄 宋姗珊 吴云秋 刘喜慧 姚彩云[1] 宋梦新 刘茹欣 赵以民[1] 杨晓男 宋志军[1,3] 阮丽君 YAN Bing-xiong;SONG Shan-shan;WU Yun-qiu;LIU Xi-hui;YAO Cai-yun;SONG Meng-xin;LIU Ru-xin;ZHAO Yi-min;YANG Xiao-nan;SONG Zhi-jun;RUAN Li-jun(National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;State Key Laboratory of Quality Research in Chinese Medicine,Macao University of Science and Technology,Macao 999078,China;Pharmaceutical College,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西壮族自治区药用植物园,西南濒危药材资源开发国家工程研究中心,广西南宁530023 [2]澳门科技大学中药质量研究国家重点实验室,中国澳门特别行政区999078 [3]广西医科大学药学院,广西南宁530021

出　　处：《中国现代中药》2024年第11期1867-1874,共8页Modern Chinese Medicine

基　　金：广西中医药适宜技术开发与推广项目(GZSY22-03);广西药用植物园科研基金项目(桂药基202001);广西药用植物园创新团队项目(桂药创2019006)。

摘　　要：目的:以不同国家(地区)穿心莲为研究对象,通过指纹图谱、化学模式识别和多成分含量测定的方法分析不同产地穿心莲质量。方法:建立26批穿心莲药材指纹图谱,进行相似度评价分析,采用化学模式识别方法对26批穿心莲药材进行主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA),寻找各产地穿心莲的差异性成分,并进行含量测定。结果:穿心莲指纹图谱有15个共有峰,除S2、S4和S15外,其余23批相似度均大于0.8;PCA将26批穿心莲药材按产地划分为2类,OPLS-DA进一步筛选出3个差异性标志物,分别是穿心莲内酯、14-去氧穿心莲内酯和脱水穿心莲内酯;含量测定结果显示,国外采集的穿心莲中二萜内酯成分普遍高于国内样品。结论:建立的指纹图谱方法结合化学模式识别能有效分析不同产地穿心莲药材质量的差异性,且国内外不同产地穿心莲中二萜内酯含量具有明显差异,为穿心莲药材质量及种植提供参考。Objective:The quality of Andrographis paniculata from different countries and regions was analyzed by fingerprint,chemical pattern recognition,and multi-component content determination.Methods:The similarity of 26 batches of A.paniculata was evaluated and analyzed with the fingerprints established in this study.Chemical pattern recognition was employed for principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)to find the differential components of A.paniculata from different producing areas,and content determination was also performed.Results:There were 15 common peaks in the fingerprints of A.paniculata.Except for the three batches of samples S2,S4,and S15,the similarity of the other 23 batches was greater than 0.8.According to PCA,the 26 batches of A.paniculata were divided into two categories.Three differentiation biomarkers were determined by OPLS-DA,namely andrographolide,14-deoxyandrographolide,and dehydroandrographolide.The content of diterpene lactone in A.paniculata from other countries was generally higher than that from China.Conclusion:The established fingerprint method combined with chemical pattern recognition can effectively analyze the quality difference of A.paniculata from different producing areas,and the diterpene lactone content of A.paniculata from China and other countries is significantly different.This study provides a reference for the quality improvement and planting of A.paniculata.

关 键 词：穿心莲 质量 指纹图谱 化学计量学 含量测定 二萜内酯 

分 类 号：R284[医药卫生—中药学]