马蔺子HPLC特征图谱和3个成分含量测定  

HPLC Characteristic Chromatogram of Iridis Semen and Content Determination of Three Components

作　　者：郭晓霞 徐宁 李冬华 雷虹 GUO Xiao-xia;XU Ning;LI Dong-hua;LEI Hong(Gansu Institute for Drug Control,Lanzhou 730070,China;NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine,Lanzhou 730070,China)

机构地区：[1]甘肃省药品检验研究院,甘肃兰州730070 [2]国家药品监督管理局中药材及饮片质量控制重点实验室,甘肃兰州730070

出　　处：《中国现代中药》2024年第11期1886-1891,共6页Modern Chinese Medicine

基　　金：兰州市科技计划项目(2022-3-41);中药材及饮片质量控制重点实验室项目(2024GSMPA-KL13,2023GSMPA-KL06);甘肃省药品监督管理局项目(2022GSMPA0017)。

摘　　要：目的:采用高效液相色谱法(HPLC)建立民族药马蔺子的特征图谱,测定其3个成分的含量。方法:以白藜芦醇、马蔺子甲素和马蔺子乙素为指标,采用Waters Atlantis T3色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,流速为1.0 mL·min~(–1),检测波长为254 nm,柱温为30℃。结果:建立马蔺子HPLC特征图谱,标定3个特征峰,55 min内马蔺子的主要色谱峰能够达到完全分离,对16批药材中3个成分进行含量测定。白藜芦醇、马蔺子甲素和马蔺子乙素质量浓度分别为0.006 08~0.121 60、0.042 66~2.133 00、0.004 82~0.241 00 mg·mL~(–1)时与峰面积线性关系良好,平均回收率分别为90.50%、100.36%、91.81%;16批马蔺子中3个成分的质量分数分别为0.156~0.369、1.774~5.709、0.090~0.203 mg·g~(–1)。结论:建立的HPLC特征图谱专属性强,结合3个主要成分的含量测定能够为马蔺子的质量控制提供参考。Objective:The high-performance liquid chromatography(HPLC)method was established to create a characteristic chromatogram for the traditional ethnic medicine Iridis Semen and to determine the content of its three components.Methods:Using resveratrol,irisquinone A,and irisquinone B as indexes,a Waters Atlantis T3 chromatographic column(250 mm×4.6 mm,5μm)was employed.The mobile phase gradient elution consisted of acetonitrile and 0.1%phosphoric acid aqueous solution,with a flow rate of 1.0 mL·min–1.The detection wavelength was set at 254 nm,and the column temperature was maintained at 30℃.The results were analyzed using Chempattern Chemometric software.Results:The HPLC characteristic chromatogram of Iridis Semen was established,calibrating three characteristic peaks.The main chromatographic peaks of Iridis Semen achieved complete separation within 55 min.The content of three components was determined in 16 batches of medicinal materials.The mass concentrations of resveratrol,irisquinone A,and irisquinone B exhibited good linear relationships with peak areas in the ranges of 0.00608-0.12160,0.04266-2.13300,and 0.00482-0.24100 mg·mL–1,respectively,with average recoveries rates of 90.50%,100.36%,and 91.81%.The mass fractions of the three components in 16 batches of Iridis Semen were 0.156-0.369,1.774-5.709,and 0.090-0.203 mg·g–1,respectively.Conclusion:The established HPLC characteristic chromatogram shows strong specificity.Combined with the content determination of the three main components,it can provide a reference for quality control of the Iridis Semen.

关 键 词：马蔺子 白藜芦醇 马蔺子甲素 马蔺子乙素 特征图谱 多组分含量测定 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]