不同程度制草乌生物碱含量对大鼠迷走神经动作电位和心电图的影响  

Effects of Content of Alkaloids in Aconiti Kusnezoffii Radix with Different Degrees ofProcessing on Vagus Nerve Action Potential and Electrocardiogram in Rats

作　　者：李文汇 高蔚 宋亮亮 祖文婷 王家胜 王宏月 王靖 赵颖 刘子琴 包丽媛 杜红[1] LI Wenhui;GAO Wei;SONG Liangliang;ZU Wenting;WANG Jiasheng;WANG Hongyue;WANG Jing;ZHAO Ying;LIU Ziqin;BAO Liyuan;DU Hong(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院,北京100029 [2]北京中医药大学中医学院,北京100029

出　　处：《中国中医药信息杂志》2024年第12期129-135,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(82274206、81774004)。

摘　　要：目的比较不同炮制程度的制草乌生物碱含量及对SD大鼠心电图、颈部迷走神经动作电位的影响,建立生草乌及其炮制品的毒性评价方法。方法采用HPLC测定生草乌及泡煮30 min、不泡煮4 h、泡煮4 h、泡煮8 h的制草乌中苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、新乌头碱、次乌头碱、乌头碱6个生物碱成分的含量;SD大鼠舌下引流分别给予草乌生品及4种炮制品,记录给药前后大鼠迷走神经动作电位及心电图,对生物碱含量与迷走神经放电面积和心率进行相关性分析。结果4种制草乌双酯型生物碱含量为:泡煮30 min>不泡煮4 h>泡煮4 h>泡煮8 h;单酯型生物碱含量为:不泡煮4 h>泡煮30 min>泡煮4 h>泡煮8 h。与给药前比较,大鼠舌下给予不同炮制品后迷走神经放电幅度增加,心率升高,心律失常程度增加,且随炮制程度而变化;相关性分析显示,迷走神经放电面积与双酯型生物碱和总生物碱含量有明显相关性。结论大鼠舌下引流给药后迷走神经动作电位和心电图可反映草乌麻舌程度,可为草乌“麻舌感”的研究提供参考。Objective To compare the contents of alkaloid in Aconiti Kusnezoffii Radix with different degrees of processing and their effects on electrocardiogram and vagus nerve action potential in SD rats;To establish a toxicity evaluation method for Aconiti Kusnezoffii Radix and its processed products.Methods HPLC was used to determine the contents of six alkaloid components,including benzoyl neoaconitine,benzoyl aconitine,benzoyl subeaconitine,neoaconitine,subeaconitine and aconitine in Aconiti Kusnezoffii Radix and Aconiti Kusnezoffii Radix Cocta of soaking and boiled for 30 minutes,boiled for 4 h without soaking,soaking and boiled for 4 h,and soaking and boiled for 8 h.SD rats were subjected to sublingual drainage with Aconiti Kusnezoffii Radix and four types of processed products.The vagus nerve action potential and electrocardiogram of rats before and after administration were recorded,and the correlation analysis between alkaloid content and vagus nerve action potential and heart rate was conducted.Results The contents of alkaloid of the four processed products showed that the content of diester alkaloids was soaking and boiled for 30 min>boiled for 4 h without soaking>soaking and boiled for 4 h>soaking and boiled for 8 h;monoester alkaloid content:boiled for 4 h without soaking>soaking and boiled for 30 min>soaking and boiled for 4 h > soaking and boiled for 8 h. After the administration of each sample, compared with thebasal discharge, the vagus nerve discharge of rats with different processed products was changed by sublingualadministration, heart rate increased, the degree of arrhythmia increased, and it varied with the degree of processing;the results of correlation analysis showed that there was a significant correlation between the nerve discharge area andthe contents of diester alkaloids and total alkaloids. Conclusion The action potential of the vagus nerve andelectrocardiogram of rats after sublingual drainage administration can reflect the degree of numbness in the tongue,which can provide reference for t

关 键 词：草乌 炮制 生物碱 电生理信号 大鼠 

分 类 号：R284.1[医药卫生—中药学] R283.1[医药卫生—中医学]