皖贝母与贝母类药材生物碱含量测定与分析  

Content Determination and Analysis of Alkaloid Contents Between Fritillariae Anhuiensis Bulbus and 5 Fritillaria Medicinal Materials

作　　者：麻泽芊 罗汉 张虹 方成武 刘守金 MA Zeqian;LUO Han;ZHANG Hong;FANG Chengwu;LIU Shoujin(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Institute of Traditional Chinese Medicine Resources Protection and Development,Anhui Academy of Chinese Medicine,Hefei 230012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽省中医药科学院中药资源保护与开发研究所,安徽合肥230012

出　　处：《中国中医药信息杂志》2024年第12期136-141,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：安徽省中医药管理局中药质量保障项目(2021年)。

摘　　要：目的分析皖贝母与5种贝母类药材的总生物碱及贝母辛等6个生物碱成分,为皖贝母药用提供依据。方法采用紫外分光光度法测定总生物碱含量,并建立超高效液相色谱-蒸发光散射检测器(UPLCELSD)同时测定皖贝母与5种贝母类药材中贝母辛、西贝母碱苷、西贝母碱、贝母甲素、贝母乙素、异浙贝甲素6个生物碱成分含量的方法,采用聚类分析和主成分分析对18批贝母类药材进行分类。结果皖贝母的总生物碱含量显著高于其他贝母,聚类分析及主成分分析将皖贝母与浙贝母和湖北贝母分为一类。结论皖贝母生物碱成分与浙贝母和湖北贝母相似,可作为药用栽培品种。Objective To analyze the total alkaloids and six alkaloid components,including peimisine,of Fritillariae Anhuiensis Bulbus and five types of Fritillaria medicinal materials;To providebasis for the medicinal use of Fritillariae Anhuiensis Bulbus.Methods The total alkaloid content and 6 alkaloid components(peimisine,sipeimine-3β-D-glucoside,sipeimine,peimine,peiminine and isoverticine)of Fritillariae Anhuiensis Bulbus and 5 medicinal Fritillaria were determined by UV-Spectrophotometry and UPLC-ELSD method,and 18 batches of Fritillaria medicinal materials were classified by clustering analysis and principal component analysis.Results The total alkaloid content of Fritillariae Anhuiensis Bulbus was significantly higher than that of other Fritillarias.The results of clustering and principal component analysis showed that Fritillariae Anhuiensis Bulbus was classified into one class with Fritillariae Thunbergii Bulbus and Fritillariae Hupehensis Bulbus.Conclusion Fritillariae Anhuiensis Bulbus is similar to Fritillariae Thunbergii Bulbus and Fritillariae Hupehensis Bulbus in the composition of alkaloids,which can be used as a medicinal cultivation variety.

关 键 词：皖贝母 药用贝母 紫外分光光度法 超高效液相色谱-蒸发光散射检测器 生物碱 

分 类 号：R284.1[医药卫生—中药学]