基于中西医临床病证特征的糖尿病胃轻瘫动物模型分析  

Analysis of Animal Models of Diabetic Gastroparesis Based on Clinical Disease-syndrome Characteristics of Traditional Chinese and Western Medicine

作　　者：韩纪元 薛姗 谭浩原 刘敏[2] 王保华[2] HAN Jiyuan;XUE Shan;TAN Haoyuan;LIU Min;WANG Baohua(Guangzhou University of Chinese Medicine,Guangzhou 510405,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院,广州510405

出　　处：《世界中医药》2024年第18期2778-2783,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(82104791)——泻热祛瘀方对糖脂毒性下B细胞去分化的调控及机制研究。

摘　　要：目的:根据糖尿病胃轻瘫(DGP)的中西医病证特征及诊断,对现有文献DGP的动物模型进行总结,剖析动物模型的特征和未来发展走向,为发展新的DGP动物模型提供思路。方法:通过文献检索归纳动物模型特征,建立中西医临床诊断标准,对模型的相关症状及指标进行赋值分析,得出其临床吻合度。结果:在纳入的文献中,造模动物中使用频数最高的是SD大鼠(114次)和Wistar大鼠(34次)。造模动物的性别以雄性为主。对动物模型赋值后,发现临床吻合度最高的造模方式是对实验动物注射链脲佐菌素(STZ)+高糖高脂饲料或灌服熟地黄水煎液,中西医临床吻合度分别为80%与95%。自发性DGP动物模型的中西医临床吻合度较低,其中最低的是ob/ob小鼠,中西医临床吻合度分别为10%与35%。DGP基因工程小鼠模型的中西医临床吻合度较低,均≤50%。结论:当前DGP动物模型主要有诱导型、自发型和基因敲除型,其中以STZ诱导破坏胰岛B细胞联合高糖高脂饮食建立DGP动物模型的造模方法应用最广、中西医临床吻合度最佳。而其他动物模型的中医临床吻合度普遍偏低,未能完整体现出中医证候特征。Objective:Based on the disease-syndrome characteristics and diagnostic criteria of diabetic gastroparesis(DGP)in both traditional Chinese medicine(TCM)and Western medicine,this study summarized existing animal models of DGP,analyzed their features,and explored future directions for model development,providing insights for the creation of new DGP animal models.Methods:By conducting a literature review,the characteristics of various animal models were summarized,and clinical diagnostic standards from both TCM and Western medicine were established.Symptoms and relevant indicators of the models were evaluated to assess their alignment with clinical manifestations.Results:Among the included studies,the most frequently used animals were Sprague-dawley(SD)rats(114 times)and Wistar rats(34 times),with male animals being the predominant choice.After assigning values to the animal models,the modeling method that most closely matched clinical presentations involved the injection of STZ combined with a high-sugar,high-fat diet or oral administration of Rehmanniae Radix Praeparata decoction,with TCM and Western medicine alignment rates of 80%and 95%,respectively.Spontaneous DGP animal models showed lower alignment with clinical characteristics in both TCM and Western medicine,with the lowest alignment found in ob/ob mice,with rates of 10%(TCM)and 35%(Western medicine).Genetically engineered DGP mouse models also demonstrated low clinical alignment,with both TCM and Western alignment rates≤50%.Conclusion:Current DGP animal models mainly include induced,spontaneous,and gene knockout types.The most widely used model,with the highest alignment to both TCM and Western clinical manifestations,is the STZ-induced model that destroys pancreaticβ-cells combined with a high-sugar,high-fat diet.Other animal models generally show lower alignment with TCM characteristics,failing to fully represent TCM syndromes.

关 键 词：糖尿病胃轻瘫 动物模型 临床吻合度 模型评价 病证结合 诊断标准 吻合度分析 整合医学 

分 类 号：R255.4[医药卫生—中医内科学] R587.2[医药卫生—中医学]