口咽拭子样品在小鼠肺炎病毒病原检测中的应用  

Mice Oral Swab Sampling Application for PVM detection

作　　者：佟巍[1] 郭智 刘芸 冯婷婷 郭建国 苏磊 孔琪[1] 向志光[1] TONG Wei;GUO Zhi;LIU Yun;FENG Tingting;GUO Jianguo;SU Lei;KONG Qi;XIANG Zhiguang(Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences,Comparative Medical Center,Peking Union Medical College,Beijing 100021,China)

机构地区：[1]中国医学科学院医学实验动物研究所北京协和医学院比较医学中心,北京100021

出　　处：《实验动物科学》2024年第5期19-22,共4页Laboratory Animal Science

基　　金：十四五国家重点研发计划(2021YFF0702802,2021YFF0702900,20232023YFF0724901)。

摘　　要：目的评价分子病原学及血清学检测方法在小鼠肺炎病毒(PVM)检测中的适用性。方法3周龄BALB/c和C57BL/6J小鼠雌雄各半,滴鼻感染PVM,在感染后1、2、3周,1、2、3月采集各实验鼠口咽拭子及尾尖血,分别应用PCR和ELISA方法进行检测。结果两品系小鼠感染PVM病毒1周后,口咽拭子PVM核酸检出率为70%~100%,随后2周和3周检出率降低,CT值增高,1个月后无检出;外周血PVM抗体1周无检出,随后2、3周检出率为80%~100%,1至3月全部检出,且PVM抗体ELISA检测吸光度持续增高,2月和3月仍维持较高的抗体水平。结论小鼠口咽拭子样品的PCR检测结合血清学检测技术,可应用于小鼠PVM的质量控制。Objective To evaluate the applicability of molecular pathogen detection and serological testing method in the detection of pneumonia virus of mice(PVM).Method BALB/c and C57BL/6J mice,half male and half female,aged 3 weeks,were infected with PVM via nasal droplets.Oropharyngeal swabs and tail tip blood were collected from each experimental mouse at 1 week,2 weeks,3 weeks,1 month,2 months,and 3 months post-infection,and were tested using PCR and ELISA method.Result One week after infection with PVM,the detection rate of PVM nucleic acid in oropharyngeal swabs of mice from both strains was 70%to 100%,which decreased and had higher CT values in the following 2 weeks and 3 weeks,with no detection after 1 month;peripheral blood PVM antibodies were not detected in the first week,but the detection rate was 80%to 100%in the following 2 weeks and 3 weeks,with all being detected from 1 to 3 months,and the optical density of PVM antibodies detected by ELISA continued to increase,maintaining a high antibody level in the 2nd and 3rd months.Conclusion PCR detection of oropharyngeal swab samples combined with serological testing technology can be applied to the quality control of PVM in mice.

关 键 词：小鼠肺炎病毒 口咽拭子 消长规律 

分 类 号：Q95-3[生物学—动物学]