改良特异性皮炎小鼠模型的建立与评估  

Establishment and Evaluation of Mouse Model of Modified Atopic Dermatitis

作　　者：武强 王延康 浮欣瑶 张茂森 李雪萍[2] 栾晶 WU Qiang;WANG Yankang;FU Xinyao;ZHANG Maosen;LI Xueping;LUAN Jing(Xi’an Academician Workstation,Institute of Basic and Translational Medicine,Xi’an Medical University,Xi’an 710021,China;Xi’an Key Laboratory for the Prevention and Treatment of Common Aging Diseases,Chronic Disease Prevention Research and Transformation Center,Institute of Basic and Translational Medicine,Xi’an Medical University,Xi’an 710021,China)

机构地区：[1]西安市院士工作站,基础与转化医学研究所,西安医学院,西安710021 [2]西安市常见老龄疾病防治重点实验室,慢病防治研究与转化中心,基础与转化医学研究所,西安医学院,西安710021

出　　处：《实验动物科学》2024年第5期23-29,共7页Laboratory Animal Science

基　　金：陕西省重点研发计划项目(2021ZDLSF02-09)。

摘　　要：目的对现有的建模方法和评价指标进行优化以提高模型的成功率和实验结果的可靠性,同时,对BALB/c和C57BL/6J两种不同品系的小鼠特异性皮炎模型进行了对比评价。方法8~10周龄BALB/c和C57BL/6J小鼠各16只,分为对照组和模型组(每组8只),使用2,4-二硝基氯苯(DNCB)诱导小鼠产生特异性皮炎(AD)样改变,动态观察并统计小鼠皮炎发病程度,抓挠次数和体质量等,在实验观察的终点收集对照组和模型组小鼠脾指数,对外周血免疫细胞变化和血清相关炎症因子水平等相关数据进行统计分析。结果实验第3天,两种品系模型组小鼠均开始出现皮肤肿胀、红斑等皮炎表型,随时间进展皮炎症状进一步加重。与同品系对照组小鼠相比,各模型组小鼠有明显的皮炎表现,脾指数、抓挠次数、外周血免疫细胞、相关炎症因子水平均高于其对照组水平。BALB/c小鼠的皮炎表型更易观察,但各指标与C57BL/6J小鼠有较大差异。结论两种品系小鼠都可用于AD小鼠模型构建。与C57BL/6J小鼠相比,BALB/c小鼠的皮炎症状更为明显,皮炎恢复能力弱。Objective To improve the model’s success rate and the reliability of the experimental result,the existing modeling method and evaluation indexes were optimized,and a comparative evaluation of two different strains of mouse-specific dermatitis models,BALB/c and C57BL/6J,was performed.Method At the age of 8-10 weeks,BALB/c and C57BL/6J mice were divided into control and model groups(n=8).2,4-dinitrochlorobenzene(DNCB)was used to induce specific dermatitis(AD)-like changes in mice.The incidence degree of dermatitis,the number of scratching and body weight of mice were dynamically observed and counted.The spleen index,peripheral blood immune cells,and serum levels of inflammatory factors were collected from control and model mice at the end of the experiment for statistical analysis.Result On the third day of the experiment,mice from both strains developed skin swelling,erythema,and other dermatitis phenotypes,which worsened as the experiment progressed.When compared to control mice of the same strain,the mice in each model group had significant dermatitis,as well as higher levels of the splenic index,scratching frequency,peripheral blood immune cells,and relevant inflammatory factors.The dermatitis phenotype was more visible in BALB/c mice,but the spleen index,scratching frequency,peripheral blood immune cells,and dermatitis score differed from C57BL/6J mice.Conclusion The two strains of mice can be used in the development of AD mouse models.However,the BALB/c mice demonstrated a greater incidence of dermatitis and displayed a slower rate of recovery than the C57BL/6J mice.

关 键 词：特异性皮炎 造模方法改良 C57BL/6J小鼠 BALB/C小鼠 

分 类 号：Q95-3[生物学—动物学]