树鼩消化系统主要组织器官组织学观察  

Histological Investigation of Digestive System in Tree Shrews

作　　者：陈玥 苏丹 贵文娟 孙效容 CHEN Yue;SU Dan;GUI Wenjuan;SUN Xiaorong(State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,China;Chengdu Bio-HT Company Limited,Chengdu 610041,China;General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]四川大学生物治疗全国重点实验室,成都610041 [2]成都合拓创展生物科技有限公司,成都610041 [3]宁夏医科大学总医院,银川750004

出　　处：《实验动物科学》2024年第5期30-35,共6页Laboratory Animal Science

基　　金：四川省科技计划(2021JDRC0061)。

摘　　要：目的观察人与正常成年树鼩消化系统主要组织器官的组织学特性,为树鼩作为人类疾病动物模型在消化系统中的应用提供组织学依据。方法正常成年树鼩6只,雌雄各半,动物安乐死后大体解剖,取材舌、食管、肝、胆囊、胰腺、胃、胃幽门腺、十二指肠和空肠组织等消化系统主要组织器官,固定后常规包埋切片进行HE染色。观察树鼩消化系统主要器官的组织学特征。结果树鼩消化系统主要器官组织学特点基本与人类似,其中舌、食管、肝、胆囊、胃、胃幽门腺、十二指肠、空肠形态结构与人相似度高。但树鼩消化系统组织中食管和胰腺与人略有差异,人食管肌层较厚,树鼩食管肌层较薄。人胰腺中胰岛形状以球形为主,树鼩胰腺中胰岛形状多变,以长梭形和椭圆形为主,球形为辅。结论树鼩消化系统主要器官与人类似,结合树鼩基因学特点,树鼩作为消化代谢系统尤其是各类病毒性肝炎和糖尿病等疾病的易感动物可广泛运用于消化系统疾病实验动物的研究。Objective To investigate the histological characteristics of the tissues and organs of the digestive system of human and normal adult tree shrews and demonstrate their potential as animal models for human diseases in the digestive system.Method Six normal adult tree shrews(half male and half female)were euthanized and subjected to dissection.The tissues and organs of the digestive system,such as the tongue,esophagus,liver,gallbladder,pancreas,gastric,gastric pyloric gland,duodenum,and jejunum,were collected and subsequently fixed.Histological characteristics of the main organs of the tree shrew’s digestive system were examined by HE staining on conventional embedded sections.Result Digestive systems of tree shrews ware highly similar to humans in terms of the histological characteristics of its main organs.There is a slight distinction,humans possess a thicker muscular layer in the esophagus whereas tree shrews have a thinner muscular layer in the esophagus.The shape of pancreatic islets in the human pancreas is mainly spherical,whereas in the tree shrew pancreas the shape of islets is variable,with long spindle and oval shapes being the primary shapes with small amount spherical shapes.Conclusion Tree shrews possess a digestive system that is analogous to that of humans,which,along with their genetic predisposition,make them a suitable species for the study of digestive system diseases such as viral hepatitis and diabetes mellitus.

关 键 词：树鼩 消化系统 肝炎 组织学 

分 类 号：Q95-3[生物学—动物学]