长爪沙鼠肾病变随年龄变化的研究  

A Study on the Changes of Renal Lesions in Long Clawed Gerbils with Age

作　　者：李梦 陈柏安 卢静[1,2,3] LI Meng;CHEN Bai’an;LU Jing(Department of Experimental Animals,Capital Medical University,Beijing 100069,China;Department of Experimental Zoology,School of Basic Medicine,Capital Medical University,Beijing 100069,China;Beijing Key Laboratory of Nerve Regeneration and Repair,School of Basic Medicine,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学实验动物部,北京100069 [2]首都医科大学基础医学院实验动物学学系,北京100069 [3]首都医科大学基础医学院神经再生修复研究北京市重点实验室,北京100069

出　　处：《实验动物科学》2024年第5期59-66,共8页Laboratory Animal Science

基　　金：新型实验动物资源库服务科技创新能力提升的关键技术研究与示范(2021YFF0702805)。

摘　　要：目的本实验通过对长爪沙鼠不同年龄段肾病理表现特征的研究,为相关肾病变研究提供基础数据支持。方法利用HE染色观察长爪沙鼠不同年龄段肾病理表现,利用CaseViewer对肾小球、肾小囊、近曲小管进行记录、分析;利用CaseViewer对肾病理评分进行分析。结果在12、48和72周时,可见肾小球增大,挤压肾小囊囊间隙;肾小球外周毛细血管扩张、充血;肾小球与肾小囊黏连,并破坏附近近曲小管;肾小囊壁层增厚、分层;48、72周时,可见近曲小管管腔内蛋白样均质红染物质和肾小球坏死;病理评分显示,随着年龄的增长,不论是雌性还是雄性,其肾病理程度都呈上升趋势。其中雄性长爪沙鼠,12与8周比较,72与48周比较,肾小体病理评分都出现极显著性增长(P<0.01);而雌性长爪沙鼠,12与8周比较,48与12周比较,72与48周比较,肾小体病理评分都出现极显著性增长(P<0.01)。结论在12~72周时,长爪沙鼠表现出广泛的肾病理损伤,并且随着年龄的增长肾病理损伤逐渐加重。Objective This study provides reference data support for related kidney disease research by studying the pathological characteristics of the kidneys in different age groups of long clawed gerbils.Method Using He staining to observe the pathological manifestations of the kidneys in different age groups of long clawed gerbils,and using CaseViewer to record and analyze the glomeruli,renal vesicles,and proximal tubules;analyzing renal pathology scores using CaseViewer.Result At 12,48,and 72 weeks,glomerulus enlargement can be observed,squeezing the space between renal sacs;dilation and congestion of peripheral capillaries in the glomerulus;adhesion between glomeruli and renal vesicles,and destruction of nearby convoluted tubules;thickening and stratification of the renal capsule wall layer.At 48 and 72 weeks,protein like homogeneous red staining and glomerular necrosis were observed in the lumen of the proximal tubules;the pathological score showed an increasing trend with age in both females and males.Among them,male long clawed gerbils showed a highly significant increase in renal corpuscle pathological score between 12 and 8 weeks,and 72 and 48 weeks(P<0.01),while female long clawed gerbils showed a highly significant increase in renal corpuscle pathological score between 12 and 8 weeks,48 and 12 weeks,and 72 and 48 weeks(P<0.01).Conclusion At 12-72 weeks,the renal pathology of long clawed gerbils showed extensive renal pathological damage,and the renal pathological damage gradually worsened with age.

关 键 词：长爪沙鼠 衰老-肾病变 肾小球 肾小囊 近曲小管 

分 类 号：Q95-3[生物学—动物学]