“肺俞”“天枢”艾灸对支气管哮喘模型大鼠气道重塑、免疫细胞分化及肠道短链脂肪酸的影响  

Effects of Moxibustion at "Feishu (BL13)" and "Tianshu (ST25)" on Airway Remodeling,Immune Cell Differentiation and Intestinal Short-chain Fatty Acids in Bronchial Asthma Model Rats

作　　者：任玲 周竞颖 来奕恬 屈艺卓 张国山[1] REN Ling;ZHOU Jingying;LAI Yitian;QU Yizhuo;ZHANG Guoshan(School of Acupuncture,Moxibustion,Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha,410208)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南省长沙市410208

出　　处：《中医杂志》2024年第21期2240-2249,共10页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81603705);湖南省自然科学基金(2021JJ30513);湖南省教育厅科学研究项目(20B444);中国博士后科学基金(2017M612567)。

摘　　要：目的 探讨艾灸肺俞、天枢防治支气管哮喘的可能作用机制。方法 48只SD大鼠随机分为正常组12只、造模组36只。造模组大鼠采用卵清白蛋白(OVA)腹腔及皮下注射致敏联合雾化激发的方法构建支气管哮喘大鼠模型。将造模成功的32只大鼠随机分为模型组、肺俞组、天枢组和肺俞-天枢组,每组8只。正常组和模型组大鼠捆绑固定不做干预,肺俞组大鼠艾灸双侧“肺俞”30 min,天枢组大鼠艾灸双侧“天枢”30 min,肺俞-天枢组大鼠艾灸双侧“肺俞”和“天枢”各15 min。艾灸干预结束1 h后,除正常组外其余各组大鼠均给予1%OVA溶液雾化激发20 min,正常组用同体积0.9%氯化钠溶液雾化20 min。以上干预措施均每天1次,连续14天。造模后和干预期间进行行为学观察,末次干预24 h后取材,采用HE染色、Masson染色分别观察各组大鼠肺组织病理学形态变化,计算胶原纤维沉积面积百分比;ELISA法检测血清中白细胞分化抗原11b (CD11b)、白细胞分化抗原40 (CD40)、白细胞分化抗原86 (CD86)、程序性死亡配体2 (PD-L2)含量,以及肺组织中白细胞介素8 (IL-8)、白细胞介素11 (IL-11)、白细胞介素27 (IL-27)含量;Western blot法检测肺组织中基质金属蛋白酶9 (MMP-9)、基质金属蛋白酶抑制剂1 (TIMP-1)、转化生长因子β(TGF-β)蛋白表达水平;采用气相色谱-质谱联用检测器检测大鼠粪便中6种(乙酸、丙酸、异丁酸、丁酸、戊酸、正己酸)短链脂肪酸(SCFAs)含量。结果 与正常组比较,模型组大鼠逐渐出现精神萎靡或烦躁不安,毛发枯槁暗淡,活动迟缓,进食量减少,大便不成形,伴有呼吸急促、喘息症状;病理学形态结果显示肺组织结构重度异常,支气管周围可见大量炎症细胞浸润,气道平滑肌层增厚以及大量胶原纤维沉积,胶原纤维沉积面积百分比增加;大鼠血清中CD11b、CD40、CD86、PD-L2含量均升高,肺组织中IL-8、IL-11、IL-27含量和MMP-Objective To explore the possible mechanism of moxibustion at"Feishu(BL13)"and"Tianshu(ST25)"in treatment of bronchial asthma.Methods A total of 48 SD rats were randomly divided into normal group(n=12)and modeling group(n=36).The bronchial asthma rat model was established by sensitization with ovalbumin(OVA)injection and aerosol provocation.Thirty-two successfully modeled rats were further randomly divided into four groups including model group,Feishu group,Tianshu group,and Feishu-Tianshu group,with 8 rats in each group.Rats in the normal group and the model group were tied and fixed without intervention,while those in the Feishu group received moxibustion at bilateral of"Feishu(BL13)"for 30 minutes;rats in the Tianshu group received moxibustion at bilateral"Tianshu(ST25)"for 30 minutes,and those in the Feishu-Tianshu group received moxibustion at both"Feishu(BL13)"and"Tianshu(ST25)"bilaterally for 15 minutes each.One hour after the intervention,1%OVA solution was aerosolized for 20 minutes in all groups except the normal group,which was given the same volume of 0.9%sodium chloride solution instead of OVA solution for aerosol stimulation.The above interventions were performed once daily for 14 days.Behavioral observations were performed after modeling and during the interventions.The samples were collected 24h after the last intervention.HE and Masson staining were used to observe pathological morphological changes of lung tissues,and the percentage of collagen fiber deposition area was counted.The levels of leukocyte differentiation antigen11b(CD11b),leukocyte differentiation antigen 40(CD40),leukocyte differentiation antigen 86(CD86),and programmed death ligand 2(PD-L2)in serum,as well as the expression of interleukin-8(IL-8),interleukin-11(IL-11),interleukin-27(IL-27)in lung tissue,were detected by enzyme-linked immunosorbent assay(ELISA).Western blot was used to detect the expression of matrix metalloproteinase-9(MMP-9),matrix metalloproteinase inhibitor 1(TIMP-1)and transforming growth factorβ(TGF-β)prote

关 键 词：支气管哮喘 艾灸 肺俞 天枢 气道重塑 免疫细胞分化 短链脂肪酸 炎症因子 肺肠同治 

分 类 号：R245[医药卫生—针灸推拿学]