LC-MS/MS法同时测定鹿角片中12种核苷的含量  

Simultaneous content determination of twelve nucleosides in Cervi Cornu slices by LC-MS/MS

作　　者：时潇丽 方冰倩 刘伟[1] 沈杰 刘瑾[1] 刘力[1] SHI Xiao-li;FANG Bing-qian;LIU Wei;SHEN Jie;LIU Jin;LIU Li(Department of Pharmacy,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;College of Pharmacy,Shanghai University of Medicine and Health Sciences,Shanghai 201308,China)

机构地区：[1]上海中医药大学附属曙光医院药学部,上海201203 [2]上海健康医学院药学院,上海201308

出　　处：《中成药》2024年第11期3552-3557,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金青年项目(82104429);国家自然科学基金面上项目(81573648)。

摘　　要：目的建立LC-MS/MS法同时测定鹿角片中鸟嘌呤、腺苷、2′-脱氧尿苷、尿苷、尿嘧啶、胸腺嘧啶、胞苷、黄嘌呤、次黄嘌呤、鸟苷、肌苷、β-胸苷的含量。方法分析采用Waters X select HSS T3色谱柱(2.1 mm×100 mm,5μm);流动相甲醇-0.1%甲酸,梯度洗脱;体积流量200μL/min;柱温30℃;电喷雾离子源;正离子扫描;多反应监测模式。结果12种核苷在各自范围内线性关系良好(r≥0.9990),平均加样回收率92.46%~104.16%,RSD 2.92%~6.14%。结论该方法稳定可行,可用于鹿角片的质量控制。AIM To establish an LC-MS/MS method for the simultaneous content determination of guanine,adenosine,2′-deoxyuridine,uridine,uracil,thymine,cytidine,xanthine,hypoxanthine,guanosine,inosine and thymidine in Cervi Cornu slices.METHODS The analysis was performed on a 30℃thermostatic Waters X select HSS T3 column(2.1 mm×100 mm,5μm),with the mobile phase comprising of methanol and 0.1%formic acid flowing at 200μL/min in a gradient elution manner,and electron spray ionization source was adopted in positive ion scanning with multiple reaction monitoring mode.RESULTS Twelve nucleosides showed good linear relationships within their own ranges(r≥0.9990),whose average recoveries were 92.46%-104.16%with the RSDs of 2.92%-6.14%.CONCLUSION This stable and feasible method can be used for the quality control of Cervi Cornu slices.

关 键 词：鹿角片 核苷 含量测定 LC-MS/MS 

分 类 号：R284.1[医药卫生—中药学]