LC-MS/MS法同时测定连花清瘟胶囊中8种成分  

Simultaneous determination of eight constituents in Lianhua Qingwen Capsules by LC-MS/MS

作　　者：秦飘然 田佳业 李素霞[3] 高凡 余文华 刘兴超 郭秋红 QIN Piao-ran;TIAN Jia-ye;LI Su-xia;GAO Fan;YU Wen-hua;LIU Xing-chao;GUO Qiu-hong(Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Higher Education Institute Applied Technology Research Center for TCM Development and Industrialization,Shijiazhuang 050200,China;Hebei Chemical and Pharmaceutical College,Shijiazhuang 050026,China;Hebei Technology Innovation Center for TCM Formula Preparations,Shijiazhuang 050200,China)

机构地区：[1]河北中医药大学,河北石家庄050200 [2]河北省高校中药开发与产业化应用技术研发中心,河北石家庄050200 [3]河北化工医药职业技术学院,河北石家庄050026 [4]河北省中药组方制剂技术创新中心,河北石家庄050200

出　　处：《中成药》2024年第11期3564-3568,共5页Chinese Traditional Patent Medicine

基　　金：河北省科技厅重点研发计划民生科技专项(20277712D)。

摘　　要：目的建立LC-MS/MS法同时测定连花清瘟胶囊中连翘苷、连翘酯苷A、绿原酸、新绿原酸、苦杏仁苷、大黄素、大黄酸、红景天苷的含量。方法分析采用ACQUITY UPLC■ HSS T3色谱柱(100 mm×2.1 mm,1.8μm);流动相0.1%甲酸-乙腈,梯度洗脱;体积流量0.3 mL/min;柱温35℃;电喷雾离子源;负离子扫描;多反应监测模式。结果8种成分在各自范围内线性关系良好(r≥0.9995),平均加样回收率99.20%~100.96%,RSD 0.62%~1.23%。结论该方法简便、灵敏、可靠,可用于连花清瘟胶囊的质量控制。AIM To establish an LC-MS/MS method for the simultaneous content determination of forsythin,forsythoside A,chlorogenic acid,neochlorogenic acid,amygdalin,emodin,rhein and salidroside in Lianhua Qingwen Capsules.METHODS The analysis was performed on a 35℃thermostatic ACQUITY UPlC-HSS T3 column(100 mm×2.1 mm,1.8μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Eight constituents showed good linear relationships within their own ranges(r≥0.9995),whose average recoveries were 99.20%-100.96%with the RSDs of 0.62%-1.23%.CONCLUSION This simple,sensitive and reliable method can be used for the quality control of Lianhua Qingwen capsules.

关 键 词：连花清瘟胶囊 化学成分 LC-MS/MS 

分 类 号：R927.2[医药卫生—药学]