毛红椿枝叶化学成分及其抗肿瘤活性研究  

Chemical constituents from the branches and leaves of Toona ciliata var pubescens and their antitumor activities

作　　者：刘舸舟 潘卫东 李金玉 娄华勇 刘翰飞 林开琴 LIU Ge-zhou;PAN Wei-dong;LI Jin-yu;LOU Hua-yong;LIU Han-fei;LIN Kai-qin(College of Pharmacy,Guizhou Medical University/State Key Laboratory of Functions and Applications of Medicinal Plants,Guiyang 550014,China;School of Pharmaceutical Sciences,Guizhou University,Guiyang 550025,China;Guizhou Natural Products Research Center,Guiyang 550899,China)

机构地区：[1]贵州医科大学药学院/省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [2]贵州大学药学院,贵州贵阳550025 [3]贵州省天然产物研究中心,贵州贵阳550899

出　　处：《中成药》2024年第11期3676-3682,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(32160102);贵州省科技支撑项目(黔科合支撑[2022]重点019,黔科合支撑[2022]一般125);省部共建药用植物功效与利用国家重点实验室开放课题(FAMP202103K)。

摘　　要：目的研究毛红椿枝叶化学成分及其抗肿瘤活性。方法采用硅胶、RP-18反相硅胶、Sephadex LH-20及半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法测定其抗肿瘤活性。结果从中分离得到15个化合物,分别鉴定为toonaolide D(1)、toonaciliatin E(2)、bourjotinolone A(3)、(21 R,23R)-epoxy-21α-ethoxy-24 S,25-dihydroxyapotirucalla-7-en-3-one(4)、(Z)-toonasterone C(5)、(E)-toonasterone C(6)、3-epi-dyscusin C(7)、(Z)-aglawone(3)(8)、(E)-volkendousin(9)、8(14),15-isopimaradiene-2α,3α,19-triol(10)、(-)-loliolide(11)、cyclohexenone(12)、pubinernoid A(13)、山柰酚-3-O-α-L-鼠李糖苷(14),5-羟甲基-2-呋喃甲醛(15)。化合物3、4对K562细胞的IC 50值分别为54.2、47.3μmol/L,对HEL细胞的IC 50值分别为47.3、61.1μmol/L。结论化合物4、7、10、11为首次从楝科植物中分离得到,化合物2、15为首次从该植物中分离得到。化合物3和4具有较弱的抗肿瘤活性。AIM To study the chemical constituents from the branches and leaves of Toona ciliata Roem.var.pubescens(Franch.)Hand-Mazz.and their antitumor activities.METHODS The compounds were isolated and purified by silica gel,RP-18 reverse phase silica gel and semi-preparative HPLC,the structures of compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT method.RESULTS Fifteen compounds were isolated and identified as toonaolide D(1),toonaciliatin E(2),bourjotinolone A(3),(21 R,23R)-epoxy-21α-ethoxy-24 S,25-dihydroxyapotirucalla-7-en-3-one(4),(Z)-toonasterone C(5),(E)-toonasterone(6),3-epi-dyscusin C(7),(Z)-aglawone(8),(E)-volkendousin(9),8(14),15-isopimaradiene-2α,3α,19-triol(10),(-)-loliolide(11),cyclohexenone(12),pubinernoid A(13),quercetin-3-O-(4″-methoxy)-α-L-rahmnopyranosyl(14),5-hydroxymethyl-2-furancarboxaldehyde(15).The IC 50 values of compounds 3 and 4 on K562 cells were 54.2 and 47.3μmol/L,respectively,and the IC 50 values on HEL cells were 47.3 and 61.1μmol/L,respectively.CONCLUTION Compounds 4,7,10 and 11 are isolated from Toona genus for the first time,and compounds 2,15 are first isolated from this plant.Compounds 3 and 4 show weak antitumor activities.

关 键 词：毛红椿 枝叶 化学成分 分离鉴定 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]