番茄重要性状功能基因的分子标记检测及应用  

Detection and Application of Molecular Markers for Functional Genes of Important Traits in Tomato

作　　者：史茜茜 逯晓楠 李仁 李森[1] 孙帅[2] 崔霞 SHI Qianqian;LU Xiaonan;LI Ren;LI Sen;SUN Shuai;CUI Xia(College of Horticulture,Shanxi Agricultural University,Taigu 030801,Shanxi,China;State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]山西农业大学园艺学院,山西太谷030801 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081

出　　处：《中国蔬菜》2024年第11期27-38,共12页China Vegetables

基　　金：国家自然科学基金重点国际合作研究项目(32120103010);国家自然科学基金青年科学基金项目(32102403);北京市种质创制和品种选育联合攻关项目(G20220628003-03)。

摘　　要：为实现番茄重要农艺性状的分子标记辅助选择(MAS),本研究分别针对番茄抗病性(Ty-1、Ty-2、Ty-3a、Ty-5、Sm、Rx4、Tm-2、Pto、I-1、Frl、Mi-1、Bwr-12、Ph-3)、糖酸含量(STP、LIN5、ALMT9)、果实硬度(FIS1)、果形(OVATE)、花序分枝(J2、EJ2、STM3)及自封顶(SP)等性状和基因,开发了22个分子标记,对标记的分型鉴定表明,22个标记均能对番茄杂合和两种纯合基因型进行准确分型;对27份番茄商品种进行基因型鉴定,结果发现供试番茄品种中90%都含有5种及以上抗病基因,且有16份品种聚合7~9种抗病基因;6份番茄品种含高糖酸且多花序分枝基因。针对其中8个关键基因,开发了更高效的KASP标记并进行基因型检测,验证了分子标记的稳定性。综上,本试验开发的分子标记可对基因型进行准确分型,在番茄分子标记辅助选择中具有重要应用价值。To achieve high-throughput genotyping and molecular marker-assisted selection(MAS)in tomato,22 molecular markers related to tomato disease resistance(Ty-1,Ty-2,Ty-3a,Ty-5,Sm,Rx4,Tm-2,Pto,I-1,Frl,Mi-1,Bwr-12,Ph-3),sugar and acid content(STP,Lin5,ALMT9),fruit hardness(FIS1),fruit shape(OVATE),inflorescence branch(J2,EJ2,STM3)and plant type(SP)were developed.The results of genotyping showed that 22 markers could accurately classify heterozygous and two homozygous genotypes.For disease resistance,the genotypes of 27 tomato varieties showed that 90%of the tested varieties contained five or more disease resistance genes,and 16 varieties contained 7-9 disease resistance genes.Six tomato varieties contained high sugar,high acid and multi-inflorescence branching genes.In addition,efficient KASP markers were developed and genotyped for eight of these key genes to verify the stability of the molecular markers.In conclusion,the molecular markers developed in this study have important application value for improving the efficiency of resistance breeding and quality breeding in tomato and for accelerating the breeding of new varieties with high yield,high quality and disease resistance.

关 键 词：番茄 抗病性 品质 分子标记辅助选择 

分 类 号：S641.2[农业科学—蔬菜学]