黄瓜病程相关蛋白CsPRp27基因的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of Pathogenesis-related Protein Gene CsPRp27 in Cucumber

作　　者：董科 萨日娜 阳熙 刘易龙 国珺则 王新宇 张帆[1] 王立钊 DONG Ke;SA Rina;YANG Xi;LIU Yilong;GUO Junze;WANG Xinyu;ZHANG Fan;WANG lizhao(College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural University,Daqing 163319,Helongjiang,China)

机构地区：[1]黑龙江八一农垦大学园艺园林学院,黑龙江大庆163316

出　　处：《中国蔬菜》2024年第11期39-47,共9页China Vegetables

基　　金：2022年大学生创新训练项目(202210223021);黑龙江省八一农垦大学博士启动项目(XYB202116)。

摘　　要：病程相关蛋白(pathogenesis related proteins,PRs)是植物受外界胁迫时诱导表达的一类重要的防卫蛋白,其中PR-17是病程相关蛋白家族中的新成员。本研究采用RT-PCR技术从抗黑斑病黄瓜品系D1322叶片中克隆基因CsPRp27,并对其进行了生物信息学分析。结果表明:CsPRp27开放阅读框(ORF)长度为750 bp,编码260个氨基酸,为稳定的碱性分泌蛋白,属于BSP(basic secretory proteins)家族蛋白,第37~59氨基酸区域含有一个跨膜螺旋区。系统发育分析结果指出CsPRp27与甜瓜CmPRp27属同一个分支,与烟草、马铃薯、辣椒和葡萄的亲缘关系较近,且具有相同的蛋白基序motif 1~motif 5。依据启动子序列与蛋白互作结果推测CsPRp27的表达可能受茉莉酸甲酯(methyl jasmonate,MeJA)调控及组蛋白修饰。研究结果为深入揭示CsPRp27在黄瓜抗黑斑病中的功能奠定了理论基础。Pathogenesis-related proteins(PRs)were important defense protein of plants,induced in responses to different environmental stresses.PR-17 was a new member of the PRs family.In this study,CsPRp27 was cloned from resistance cucumber D1322 leaves by reverse transcription-polymerase chain reaction(RT-PCR)and its bioinformatics was analyzed.Results showed that the length of open reading frame of CsPRp27 was 750 bp and encoded 260 amino acids,belonged to member of basic secretory proteins family.CsPRp27 was a stable,basic,secretory protein,and contained a transmembrane domain between 37th and 59th amino acids.Phylogenetic tree analysis showed that CsPRp27 and CmPRp27(melon)falled into the same branch,and had close genetic relationship with Nicotiana tabacum,Solanum tuberosum,Capsicum annuum and Vitis vinifera,which were had similar protein motif 1-motif 5.According to promoter sequence and interaction protein prediction,expression of CsPRp27 probably regulated by MeJA and histone modification.The result was a foundation for further exploring the function of CsPRp27 in cucumber in resistance to plant disease.

关 键 词：黄瓜 CsPRp27基因 克隆 生物信息学分析 

分 类 号：S642.2[农业科学—蔬菜学]