不同抗性辣椒品种响应ChiVMV侵染过程中的生理生化变化  

Physiological and Biochemical Changes of Different Resistant Pepper Varieties in Response to ChiVMV Infection

作　　者：黄春梓 王日升[2,6] 吴星 赵虎[2,6] 王萌 王立浩[3] 赵曾菁 汤亚飞[4] 何志 李宗俊[2,6] 龚明霞 何龙飞[1,5] HUANG Chunzi;WANG Risheng;WU Xing;ZHAO Hu;WANG Meng;WANG Lihao;ZHAO Zengjing;TANG Yafei;HE Zhi;LI Zongjun;GONG Mingxia;HE Longfei(Agricultural College,Guangxi University,Nanning 530004,Guangxi,China;Institute of Vegetable Research,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China;Agricultural and Animal Husbandry Industry Development Research Institute,Guangxi University,Nanning 530004,Guangxi,China;Guangxi Key Laboratory of Vegetable Breeding and New Technology Development,Nanning 530007,Guangxi,China)

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西农业科学院蔬菜研究所,广西南宁530007 [3]中国农业科学院蔬菜花卉研究所,北京100081 [4]广东省农业科学院植物保护研究所,广东广州510640 [5]广西大学农牧产业发展研究院,广西南宁530004 [6]广西蔬菜育种与新技术研究重点实验室,广西南宁530007

出　　处：《中国蔬菜》2024年第11期64-73,共10页China Vegetables

基　　金：国家自然科学基金(32060687);广西重点研发计划项目(桂科AB23026024);广西农科院基本科研业务专项(桂农科2019M30,桂农科2024YP085)。

摘　　要：辣椒脉斑驳病毒(ChiVMV)是辣椒生产中危害较为严重的RNA病毒。为了明确不同抗性辣椒品种在响应ChiVMV侵染过程中的生理生化变化,以感病品种桂椒12号和抗病品种Perennial为材料,在人工摩擦接种ChiVMV后的第2、4、8、15、20天采样测定叶片中防御酶活性、叶绿素(Chl)、丙二醛(MDA)及渗透调节物质含量的变化,并调查接种后第30天的病情指数(DI)。结果表明:ChiVMV接种后第30天,桂椒12号DI值为79.34,达高感水平,而Perennial DI值为9.88,达高抗水平。接种ChiVMV后第2~20天,两个品种的过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)活性及MDA、脯氨酸(Pro)含量与对照(未接种ChiVMV)相比不同程度上升,而叶绿素含量下降,且抗病品种中Chl、Pro含量及POD活性均明显高于同期感病品种,MDA含量则明显低于感病品种。进一步分析发现Chl、Pro含量和POD活性与品种抗性呈正相关,MDA含量与品种抗性呈负相关,因此认为Chl、Pro、MDA含量和POD活性可以作为辣椒ChiVMV抗性评价的4个首选指标。Chilli veinal mottle virus(ChiVMV)is a RNA virus that causes serious damage in pepper production.In order to clarify differences in physiological and biochemical changes of different pepper varieties in response to ChiVMV infection,susceptible‘Guijiao No.12’and resistant‘Perennial’were inoculated with ChiVMV by artificial friction.On the days of 2,4,8,15,20 days after inoculation respectively,leaf samples were collected and used to measure defense enzyme activities,and content of chlorophyll(Chl),malondialdehyde(MDA)and osmoregulatory substances.Disease index(DI)of two pepper varieties was also investigated.The results were shown that‘Guijiao No.12’had a disease index of 79.34,reaching the high level of susceptibility to ChiVMV on the day of 30 days after inoculation.While‘Perennial’had a disease index of 9.88,reaching the high level of resistance to ChiVMV.From the 2-20 days after inoculation with ChiVMV,the activities of peroxidase(POD),catalase(CAT),polyphenol oxidase(PPO),and the content of malondialdehyde(MDA)and proline(Pro)of the two varieties increased on different degree,and the chlorophyll content decreased when compared with the control(no inoculation of ChiVMV).Meanwhile Chl and Pro content and POD activity of the resistant variety were significantly higher than that of the susceptible variety at the same period,and MDA content of the former was significantly lower than that of the latter.The results of further analysis were revealed that the content of Chl and Pro,and POD activity were positively correlated with the pepper resistance to ChiVMV,whereas MDA content was negatively correlated with the pepper resistance to ChiVMV.Therefore,it was considered that the content of Chl,Pro and MDA,and POD activity could be used as the preferred indexes for the evaluation of pepper resistance to ChiVMV.

关 键 词：辣椒 辣椒脉斑驳病毒 生理生化 抗性 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]