影响和牛冷冻胚胎移植妊娠率的因素分析  

Analysis of Factors Influencing the Pregnancy Rate of Frozen Embryo Transfer inWagyu Cattle

作　　者：郝海生[1] 杜卫华[1] 赵善江[1] 赵学明[1] 邹惠影 朱化彬[1] HAO Haisheng;DU Weihua;ZHAO Shanjiang;ZHAO Xueming;ZOU Huiying;ZHU Huabin(Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国草食动物科学》2024年第6期86-90,共5页China Herbivore Science

基　　金：国家重点研发计划子课题(2023YFD130060305);中国农业科学院科技创新工程(ASTIP-IAS06)。

摘　　要：为研究不同因素对和牛冷冻胚胎移植妊娠率的影响,采用Ovsynch方法对受体荷斯坦牛进行同期发情,共计移植受体557头。结果表明,A级和B级胚胎的移植妊娠率(57.72%,56.63%)差异不显著(P>0.05);不同黄体直径CL-1(<1.0 cm)、CL-2(1.0~1.5 cm)和CL-3(>1.5 cm)时的移植妊娠率(54.50%,59.72%和55.24%)差异也不显著(P>0.05);左侧和右侧子宫角移植妊娠率(56.92%,57.41%)差异不显著(P>0.05);子宫角前段移植妊娠率(59.04%)显著高于子宫角中段移植妊娠率(40.35%)(P<0.05);不同移植完成时间T-1(6~9 min)和T-2(10~13 min)内完成的移植妊娠率(60.65%,54.17%)显著高于T-3(14~24 min)内完成的移植妊娠率(26.67%)(P<0.05)。说明胚胎质量、移植部位和黄体直径对胚胎移植妊娠率均无显著影响,胚胎输送位置和移植完成时间对胚胎移植妊娠率有显著影响。In order to investigate the effects of different factors on the pregnancy rate of frozen embryo transfer in Wagyu cattle in vivo,the Ovsynch method was used to synchronize estrus in 557 recipients.The results showed that pregnancy rates following the transfer of grade A and B embryos were 57.72%and 56.63%,respectively(P>0.05).According to diameter of corpus luteum(CL),pregnancy rates were 54.50%,59.72%and 55.24%for CL-1(<1.0 cm),CL-2(1.0-1.5 cm)and CL-3(>1.5 cm),respectively(P>0.05).The rates of pregnancy after transfers to the left and right uterine horn were 56.92%and 57.41%,respectively(P>0.05).The pregnancy rate of anterior cornua uteri transplantation(59.04%)was significantly higher than that of middle horn transplantation(40.35%)(P<0.05).The pregnancy rates of transplants completed within T-1 and T-2(60.65%,54.17%)were significantly higher than those completed within T-3(26.67%)(P<0.05).It was concluded that there was no effect of embryo quality,diameter of CL and side of transfer,but embryo transport location and time to transfer completion had significant effects on the pregnancy rates during embryo transfer in recipients.

关 键 词：和牛 胚胎移植 黄体 子宫角 妊娠率 

分 类 号：S823.3[农业科学—畜牧学]