谷氧还蛋白(GRX)串联复制基因拮抗调控木薯干旱胁迫响应  

Tandem duplicated glutaredoxin genes antagonistically regulate drought responses in cassava(Manihot esculenta Crantz)

作　　者：熊华 江李伟 徐子寅 于晓玲 李淑霞[2,3] 赵平娟 李文彬 张秀春[2,3] 王文泉 阮孟斌[1,2,3] XIONG Hua;JIANG LiWei;XU ZiYin;YU XiaoLing;LI ShuXia;ZHAO PingJuan;LI WenBin;ZHANG XiuChung;WANG WenQuan;RUAN MengBin(National Key Laboratory for Tropical Crop Breeding,College of Tropical Agriculture and Forest,Hainan University,Haikou 571125,China;Sayan Research Institute,Chinese Academy of Tropical Agriculture Sciences,Sayan 572025,China;Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agriculture Sciences,Haikou 571101,China;Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava,Haikou 571101,China)

机构地区：[1]海南大学热带农林学院,热带作物生物育种全国重点实验室,海口571125 [2]中国热带农业科学院三亚研究院,三亚572025 [3]中国热带农业科学院热带生物技术研究所,海南省热带农业生物资源保护与利用重点实验室,海口571101 [4]农业农村部木薯种质资源保护与利用重点实验室,海口571101

出　　处：《中国科学：生命科学》2024年第10期1965-1973,共9页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:32272037);海南省自然科学基金(批准号:322MS146);农业农村部木薯种质资源保护与利用重点实验室项目(批准号:1630052023013)资助。

摘　　要：CC类谷氧还蛋白基因是陆生植物特有的基因家族,在植物生长发育和环境响应中起着重要的调控作用.串联复制可导致新基因产生,是CC类谷氧还蛋白基因家族扩张的一种重要方式.木薯CC类谷氧还蛋白基因家族在1号染色体上存在两个串联复制基因MeGRXC3和MeGRXC4.亚细胞定位分析表明,MeGRXC3与MeGRXC4蛋白均可定位于细胞核和细胞质中.过表达MeGRXC3的转基因木薯对甘露醇和PEG6000敏感,而过表达MeGRXC4的转基因木薯对甘露醇和PEG6000耐受.基因表达分析结果显示,MeGRXC3与MeGRXC4拮抗调控多个胁迫响应基因的表达,包括一些关键的转录因子.因此,MeGRXC3与MeGRXC4通过拮抗调控胁迫相关转录因子的表达从而调节木薯干旱胁迫响应,为抗旱木薯品种培育提供基因资源和育种策略.CC-type glutaredoxins(GRXs)are a plant specific gene family,and play important regulatory roles in plant growth,development,and stress responses.Gene tandem duplication leads to the production of new genes,which is an important way of expanding the CC-type GRX family.Two CC-type GRX tandem duplicated genes,namely MeGRXC3 and MeGRXC4,were identified in the chromosome 1 of cassava.Subcellular localization analysis showed that both MeGRXC3 and MeGRXC4 proteins were localized in the nucleus and cytoplasm of cassava.Transgenic cassava overexpressing MeGRXC3 was sensitive to D-mannitol and PEG6000,while transgenic cassava overexpressing MeGRXC4 was tolerant to these stresses.MeGRXC3 and MeGRXC4 antagonistically regulated the expression of multiple stress response genes,including some key transcription factors.Therefore,MeGRXC3 and MeGRXC4 are involved in the response of cassava to drought stress by regulating the expression of stress-related transcription factors antagonistically,which provides genetic resources and breeding strategies for cassava varieties with drought resistance.

关 键 词：木薯 CC类谷氧还蛋白 串联复制 拮抗调控 干旱 

分 类 号：Q943.2[生物学—植物学] S533[农业科学—作物学]