Establishment of High-sensitivity Rapid Fluorescence Quantitative Detection Method for Antibody against Peste des Petits Ruminants Virus  

作　　者：Zhao LIU Bo LIU Zhida LIN Hang SUN Yu SUN Xiaohui SONG 

机构地区：[1]Dalian Customs,Dalian,Liaoning 116000,China [2]Changchun Customs,Changchun 130000,China [3]Gongbei Customs,Zhuhai 519000,China [4]China Animal Disease Control Center,Beijing 102600,China [5]Beijing University of Agriculture,Beijing 100096,China

出　　处：《Agricultural Biotechnology》2024年第5期22-27,共6页农业生物技术（英文版）

基　　金：Supported by The National Project for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500);National Mutton Sheep Industrial Technology System(CARS39).

摘　　要：[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value.

关 键 词：Peste des Petits Ruminants N protein NH fusion protein Soluble expression and purification Rapid quantitative detection 

分 类 号：S85[农业科学—兽医学]