Breeding of Anti-diarrhea Gene in Local and Exotic Pig Breeds in Guizhou Province  

作　　者：Qingmeng LONG Min YAO Ping LI Wenwu FENG Mingzong TANG Jinrong SHEN Jin ZENG Junwei ZHANG Yunsong ZHANG 

机构地区：[1]Guizhou Provincial Breeding Livestock and Poultry Germplasm Testing Center,Guiyang 550018,China

出　　处：《Agricultural Biotechnology》2024年第5期33-36,共4页农业生物技术（英文版）

基　　金：Supported by Guizhou Provincial Department of Agriculture and Rural Affairs Project(QNYZZZ[2017]No.12);2024 Quality Supervision and Sampling Project of Normal Temperature Semen for Breeding Pigs.

摘　　要：[Objectives]This study was conducted to breed special pig breeds resistant to diarrhea by using modern biotechnology.[Methods]From Guizhou local breeds,such as Nuogu pigs,Kele pig,Yorkshire pigs and Duroc pigs,190 samples were collected for the analysis of anti-diarrhea gene.[Results]The anti-diarrhea genotype frequency of Kele pigs was 70.00%,which was higher than that of Nuogu pigs(67.37%)and Yorkshire pigs(Yorkshire pigs and Duroc pigs)(50.59%).The favorable anti-diarrhea gene of all Nuogu pigs,Kele pigs,and Yorkshire pigs and Duroc pigs was G,with gene frequencies of 0.7355,0.8368 and 0.8500,respectively,and the frequencies of allele A were 0.2645,0.1632 and 0.1500,respectively.In the process of generation selection,combination selection of GG♂×GG♀,GG♂×GA♀,GA♂×GG♀and GA♂×GA♀was conducted,and GG individuals were selected while gradually phasing out GA and AA individuals.The anti-diarrhea genotypes of 98 pigs in the offspring were tested,and it was found that the frequency of genotype GG was greatly improved,and the frequencies in Nuogu pigs,Kele pigs,Yorkshire pigs and Duroc pigs were increased to 73.91%,81.82%,85.25%and 66.67%respectively,thus forming a special anti-diarrhea breed.[Conclusions]This study provides a basis for selecting excellent breeding pigs,establishing core populations and screening resistance genes in the core populations and their offspring.

关 键 词：PIG ANTI-DIARRHEA MUC13 gene Generation selection New breed 

分 类 号：S856.4[农业科学—临床兽医学]