A multi-center study on genetic variations in the fusion protein of respiratory syncytial virus from children with Acute Lower Respiratory Tract Infections in China during 2017-2021  

作　　者：Yiliang Fu Fei Li Yun Zhu Luci Huang Qiuping Li Hanwen Zhang Lili Zhong Hailin Zhang Zheng-xiu Luo Gen Lu Jikui Deng Lingfeng Cao Ying Wu Rong Jin Lei Li Lili Xu Xiangpeng Chen Zhengde Xie 

机构地区：[1]Beijing Key Laboratory of Pediatric Respiratory Infection Diseases,Key Laboratory of Major Diseases in Children,Ministry of Education,National Clinical Research Center for Respiratory Diseases,National Key Discipline of Pediatrics(Capital Medical University),Beijing Pediatric Research Institute,Beijing Children's Hospital,Capital Medical University,National Center for Children's Health,Beijing 100045,China [2]Research Unit of Critical Infection in Children,Chinese Academy of Medical Sciences,Beijing 100045,China [3]Hunan Provincial People's Hospital,The First Affiliated Hospital of Hunan Normal University,Changsha 410005,China [4]Department of Children's Respiration Disease,The Second Affiliated Hospital&Yuying Children's Hospital,Wenzhou Medical University,Wenzhou 325027,China [5]Department of Respiratory Medicine,National Clinical Research Center for Child Health and Disorders,Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing Key Laboratory of Pediatrics,Children's Hospital of Chongqing Medical University,Chongqing 400015,China [6]Guangzhou Women and Children's Medical Center,Guangzhou 510623,China [7]Department of Infectious Diseases,Shenzhen Children's Hospital,Shenzhen 518026,China [8]Department of Clinical Laboratory,Children's Hospital of Fudan University,Shanghai 201102,China [9]Department of Clinical Laboratory Medicine,National Children's Medical Center,Shanghai Children's Medical Center,School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China [10]Guiyang Maternal and Child Health Hospital,Guiyang 550003,China [11]Yinchuan Maternal and Child Health Care Hospital,Yinchuan 750001,China

出　　处：《Virologica Sinica》2024年第5期727-736,共10页中国病毒学（英文版）

基　　金：supported by the National Key Research and Development Program of China(grant number 2023YFC2306002);National Science and Technology Major Projects(grant number 2017ZX10104001-005-010);CAMS Innovation Fund for Medical Sciences(CIFMS)(Grant Number 2019-I2M-5-026);Funding for Reform and Development of Beijing Municipal Health Commission.

摘　　要：Respiratory syncytial virus(RSV)is a significant cause of acute lower respiratory tract infection(ALRTI)in children underfive years of age.Between 2017 and 2021,396 complete sequences of the RSV F gene were obtained from 500 RSV-positive throat swabs collected from ten hospitals across nine provinces in China.In addition,151 sequences from China were sourced from GenBank and GISAID,making a total of 549 RSV F gene sequences subjected to analysis.Phylogenetic and genetic diversity analyses revealed that the RSV F genes circulating in China from 2017 to 2021 have remained relatively conserved,although some amino acids(AAs)have undergone changes.AA mutations with frequencies10%were identified at six sites and the p27 region:V384I(site I),N276S(site II),R213S(siteØ),and K124N(p27)for RSV A;F45L(site I),M152I/L172Q/S173 L/I185V/K191R(site V),and R202Q/I206M/Q209R(siteØ)for RSV B.Comparing mutational frequencies in RSV-F before and after 2020 revealed minor changes for RSV A,while the K191R,I206M,and Q209R frequencies increased by over 10%in RSV B.Notably,the nirsevimab-resistant mutation,S211N in RSV B,increased in frequency from 0%to 1.15%.Both representative strains aligned with the predicted RSV-F structures of their respective prototypes exhibited similar conformations,with low root-mean-square deviation values.These results could provide foundational data from China for the development of RSV mAbs and vaccines.

关 键 词：Human respiratory syncytial virus(RSV) CHILDREN Fusion glycoprotein Antigenic epitope VARIATION 

分 类 号：R725.6[医药卫生—儿科]