Microlunatus phosphovorus与Tetrasphaera的葡萄糖代谢特征及其在生物除磷过程中的协同与竞争机制研究  

Glucose metabolic characteristics of Microlunatus phosphovorus and Tetrasphaera,and their synergistic and competitive mechanisms in the enhanced biological phosphorus removal process

作　　者：黄榆珩 邱林清 王岑超 徐轩 陆凌楠 陈丽萍 谢晓婧 韦朝海 邱光磊[1,3,4] HUANG Yuheng;QIU Linqing;WANG Cenchao;XU Xuan;LU Lingnan;CHEN Liping;XIE Xiaojing;WEI Chaohai;QIU Guanglei(School of Environment and Energy,South China University of Technology,Guangzhou 510006;Dongguan Water Group Water Purification Co.,Ltd.,Dongguan 523000;Key Laboratory of Pollution Control and Ecological Restoration in Industrial Clusters,Ministry of Education,Guangzhou 510006;Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling,Guangzhou 510006)

机构地区：[1]华南理工大学环境与能源学院,广州510006 [2]东莞市水务集团净水有限公司,东莞523000 [3]工业聚集区污染控制与生态修复教育部重点实验室,广州510006 [4]固体废物污染控制与资源化广东省重点实验室,广州510006

出　　处：《环境科学学报》2024年第10期218-226,共9页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.52270035,51808297);广东省自然科学基金(No.2021A1515010494);广东省珠江人才计划(No.2019QN01L125);广州市重点研发计划(No.2023B03J1334)。

摘　　要：本研究旨在探究强化生物除磷(Enhanced Biological Phosphorus Removal,EBPR)工艺中两种常见的聚磷菌(Polyphosphate Accumulating Organisms,PAOs):Microlunatus phosphovorus(积磷小月菌)和Tetrasphaera以葡萄糖为碳源条件下的代谢特征与竞争机制.通过厌氧-好氧全周期实验,系统表征了两种PAOs的摄碳除磷动力学参数以及碳源利用与除磷的计量学关系.结果表明,在厌氧阶段,Microlunatus phosphovorus平均以109.2 mg·g^(-1)·h^(-1)的速率摄取葡萄糖(厌氧碳源摄取/释磷计量比为0.82),将葡萄糖以糖原的形式储存在细胞内,此过程中未检测到发酵产物的生成;在好氧阶段,Microlunatus phosphovorus利用在厌氧条件下储存的糖原供能吸收磷酸盐,其吸磷速率为22.6 mg·g^(-1)·h^(-1).相较之下,在厌氧阶段,Tetrasphaera的葡萄糖摄取速率明显较低(6.10 mg·g^(-1)·h^(-1),厌氧碳源摄取/释磷计量比为0.64),其将葡萄糖发酵成乙酸、琥珀酸和乳酸并排出胞外;好氧段则继续摄取葡萄糖,为吸收磷酸盐提供能量,其吸磷速率为3.7 mg·g^(-1)·h^(-1).此外,探究了Microlunatus phosphovorus和Tetrasphaera以葡萄糖为碳源条件下的相互作用特征.两者以1∶1的比例共存时,实际厌氧阶段净释磷量小于理论释磷量而葡萄糖的摄取量则大于理论值,表明两者存在碳源摄取过程中的相互促进作用.好氧阶段,两者共存可实现更低的出水磷酸盐浓度.本文通过利用葡萄糖作为唯一碳源对纯菌培养的Microlunatus phosphovorus和Tetrasphaera进行代谢研究及摄碳除磷动力学研究,构建了Microlunatus phosphovorus和Tetrasphaera以葡萄糖为碳源时的代谢模型,探究了两者的相互作用关系,研究结果将为提高以葡萄糖作为碳源条件下强化生物除磷系统运行稳定性与除磷效率提供理论支撑.This study aims to investigate the metabolic characteristics and competitive mechanisms of two commonly found polyphosphate-accumulating organisms(PAOs)-Microlunatus phosphovorus and Tetrasphaera-with glucose as a carbon source in the enhanced biological phosphorus removal(EBPR)process.Via anaerobic-aerobic cycle experiments,the study systematically characterized the carbon uptake and phosphorus removal kinetics of these two PAOs and their stoichiometric relationships between carbon source utilization and phosphorus removal.Results indicate that under anaerobic conditions,Microlunatus phosphovorus takes up glucose at an average rate of 109.2 mg·g^(-1)·h^(-1)(with a carbon uptake to phosphorus release ratio of 0.92),storing glucose as glycogen intracellularly,without the detection of any fermentation byproduct.In the subsequent aerobic phase,Microlunatus phosphovorus utilizes stored glycogen to take up phosphate at a rate of 22.6 mg·g^(-1)·h^(-1).In contrast,Tetrasphaera exhibits a significantly lower glucose uptake rate in the anaerobic phase(6.10 mg·g^(-1)·h^(-1),with a carbon uptake to phosphorus release ratio of 0.64),fermenting glucose into acetate,succinate,and lactate,excreting them extracellularly.During the subsequent aerobic phase,Tetrasphaera continues glucose uptake,providing energy for phosphate assimilation,with a uptake rate of 3.7 mg·g^(-1)·h^(-1).Moreover,this study investigated the cooperation/competitive interaction and mechanisms between Microlunatus phosphovorus and Tetrasphaera with glucose as a carbon source.When co-cultured in a 1∶1 ratio,the net phosphorus release in the anaerobic phase is less than the theoretical value;while the glucose uptake at the end of the anaerobic phase exceeds the theoretical value,suggesting a synergetic interaction between to PAO during glucose uptake.In the aerobic phase,lower effluent phosphate concentration was achieved.Via metabolic and kinetic studies on Microlunatus phosphovorus and Tetrasphaera with glucose as the sole carbon source,metabolic m

关 键 词：强化生物除磷 Microlunatus phosphovorus Tetrasphaera 摄糖除磷动力学 物质代谢机制 相互作用关系 

分 类 号：X703[环境科学与工程—环境工程] X172