南宁市主要自然水体中溶解性有机物的三维荧光光谱特征  

3D-EEM spectral characteristics of dissolved organic compounds in the main natural water bodies of Nanning

作　　者：李晓松 李文英 王宇 许雅琳 莫佳玥 许谦 董慧峪[2] 杜寒春[4] 谢婷 刘绍刚[1] LI Xiaosong;LI Wenying;WANG Yu;XU Yalin;MO Jiayue;XU Qian;DONG Huiyu;DU Hanchun;XIETing;LIU Shaogang(Guangxi Key Laboratory of Advanced Structural Materials and Carbon Neutralization,Guangxi Colleges and Universities Key Laboratory of Environmental-friendly Materials and Ecological Remediation,School of Materials and Environment,Guangxi Minzu University,Nanning 530105;Key Laboratory of Drinking Water Science and Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;Guangxi Nanning Waterworks Co.,Ltd.,Nanning 530031;Guangxi Zhuang Autonomous Region Center for Analysis and Test Research,Nanning 530022)

机构地区：[1]广西民族大学,材料与环境学院,广西先进结构材料与碳中和重点实验室,广西高校环境友好材料及生态修复重点实验室,南宁530105 [2]中国科学院生态环境研究中心,中国科学院饮用水科学与技术重点实验室,北京100085 [3]广西绿城水务股份有限公司,南宁530031 [4]广西壮族自治区分析测试研究中心,南宁530022

出　　处：《环境科学学报》2024年第11期215-229,共15页Acta Scientiae Circumstantiae

基　　金：广西科技计划项目(No.桂科AD21220003);广西科技计划项目(No.桂科AB24010225);广西民族大学研究生教育创新计划项目(No.gxun-chxs2024125)。

摘　　要：采用三维荧光光谱技术结合荧光区域积分法(FRI)分析了南宁市邕江、八尺江、大王滩水库中溶解性有机物(DOM)的荧光特性,探讨了DOM的组成、腐殖化程度、污染来源、DOM荧光组分与水质参数及微生物群落组成之间的相关性.结果表明,邕江水质符合GB3838-2002Ⅰ~Ⅱ类,大王滩水库和八尺江水质分别为Ⅱ~Ⅲ类、Ⅲ~Ⅳ类,COD_(Mn)和TP为主要污染物.3种水体中DOM均以生物来源为主,腐殖化程度较弱,新生DOM占比高,溶解性微生物代谢产物(C4)和类腐殖酸(C5)的荧光区域积分占比分别为35.2%和53.1%.八尺江和大王滩水库中DOM荧光区域积分强度明显高于邕江.大王滩水库水进入八尺江后主要微生物种群从Leptolyngbyaceae、Burkholderiaceae、Saprospiraceae变为Comamonadaceae、Cyanobiaceae、Ilumatobacteraceae,微生物群落组成明显不同于邕江,叶绿体(Chloroplast)含量较高.八尺江DOC和COD_(Mn)浓度分别与Rhodobacterace、Comamonadaceae呈负相关,NH3-N与Ilumatobacteraceae、Xanthomonadaceae呈显著正相关,TP浓度与Chloroplast、Crocinitomicaceae呈显著正相关,DOM中C4与Beijerinckiaceae有较高相关性,反映出八尺江污染与生活源和农业源排放高生物活性的DOM和氮磷有关.COD_(Mn)、TP、DOC与C4、C5具有正相关性,尤其大王滩水库COD_(Mn)、TP与C4的相关系数分别达到0.71和0.58(p<0.0001).研究结果可为三维荧光光谱技术结合FRI法应用于快速确定地表水污染来源的可行性研究提供一定参考依据.In the present work,the fluorescence characteristics of dissolved organic matter(DOM)in the Yongjiang River,Bachijiang River,and Dawangtan Reservoir in Nanning City were analyzed by three-dimensional excitation-emission matrix fluorescence spectroscopy(3D-EEM)combined with regional fluorescence integration(FRI).The DOM fluorescence components,humification degree,pollution source,and their correlations with water quality parameters,as well as microbial community,were also evaluated.The results clearly demonstrated that the water quality of Yongjiang River falls in the Class Ⅰ~Ⅱ requirements according to GB3838-2002 standards.On the other hand,the Dawangtan Reservoir and Bachijiang River were classified as Class Ⅱ~Ⅲ and Ⅲ~Ⅳ,respectively,and the COD_(Mn) and TP were identified as the primary pollutants.The DOM primary originated from biological sources and was exhibited at a low humification degree with the dissolved microbial metabolites(C4)and humic acids(C5)comprising 35.2%and 53.1%of the fluorescence integral,respectively.Note that the fluorescence integral intensity of DOM in the Bachijiang River and Dawangtan Reservoir was significantly higher than that of the Yongjiang River.As the Dawangtan Reservoir water entered the Bachijiang River,the predominant microbial community changed from Leptolyngbyaceae,Burkholderiaceae,and Saprospiraceae to Comamonadaceae,Cyanobiaceae,and Ilumatobacteraceae.The microbial community varied considerably in Yongjiang River,with a notably higher chloroplast content.It is interesting to note that a strong negative correlation was found between DOC concentration and Rhodobacterace,COD_(Mn) concentration,and Comamonadaceae in the Bachijiang River.In addition,the TP concentration was positively correlated with Chloroplast and Crocinitomycoaceae,NH3-N with Ilumatobacteraceae and Xanthomonadaceae,and C4 with Beijerinkiaceae,significantly.These correlations well suggest that pollution in the Bachijiang River may be attributed to the emissions of highly biologically active DOM a

关 键 词：地表水 三维荧光 溶解性有机物 荧光区域积分法 微生物群落 

分 类 号：X522[环境科学与工程—环境工程]