日粮中添加复合酶制剂和复合微生态制剂对AA肉鸡生长性能、血清生化指标及抗氧化能力、小肠形态发育的影响  

Effects of Dietary Supplementation of Compound Enzyme and Probiotic Preparations on Growth Performance,Serum Biochemical Indices and Antioxidant Capacity,and Small Intestinal Morphological Development of AA Broilers

作　　者：杨华 伏春燕 刘雪兰[2] 张亨 YANG Hua;FU Chunyan;LIU Xuelan;ZHANG Heng(Hunan University of Science and Engineering,Yongzhou 425199,China;Poultry Institute,Shandong Academy of Agricultural Sciences,Jinan 250023,China)

机构地区：[1]湖南科技学院,湖南永州425199 [2]山东省农业科学院家禽研究所,山东济南250023

出　　处：《畜牧与饲料科学》2024年第5期18-24,共7页Animal Husbandry and Feed Science

基　　金：济南引进创新团队项目(202228037);山东省农业科学院农业科技创新工程(CXGC2023D06);东营市科技计划项目(2020JCYJ105);山东省现代农业产业技术体系家禽创新团队建设项目(SDAIT-11-06)。

摘　　要：[目的]探究日粮中添加复合酶制剂和复合微生态制剂对AA肉鸡生长性能、血清生化指标及抗氧化能力、小肠形态发育的影响。[方法]将480只体重相近的1日龄雄性AA肉仔鸡随机分为4组,对照组饲喂玉米-豆粕型基础日粮,复合酶制剂组、复合微生态制剂组、联合添加组基础日粮中分别添加0.2 g/kg复合酶制剂、1 g/kg复合微生态制剂、0.2 g/kg复合酶制剂+1 g/kg复合微生态制剂,每组6个重复,每个重复20只。饲喂试验周期为42 d。计算各组肉鸡在不同生长阶段(1~21日龄、22~42日龄、1~42日龄)的平均日采食量(ADFI)、平均日增重(ADG)和料重比(F/G);于试验第21天和第42天,分别采集各组肉鸡血液样本,测定血清生化指标及抗氧化能力;采集各组肉鸡十二指肠、空肠、回肠样本,制备组织切片,HE染色后观察各肠段的绒毛高度(VH)和隐窝深度(CD),计算绒毛高度与隐窝深度的比值(V/C)。[结果]①与对照组相比,复合酶制剂组和联合添加组肉鸡的21日龄体重、1~21日龄ADFI和ADG、1~42日龄ADG显著(P<0.05)提高;复合酶制剂组肉鸡的22~42日龄和1~42日龄ADFI显著(P<0.05)提高,1~42日龄F/G显著(P<0.05)降低;复合微生态制剂组肉鸡的1~21日龄、22~42日龄和1~42日龄F/G显著(P<0.05)降低;联合添加组肉鸡的22~42日龄和1~42日龄F/G显著(P<0.05)降低。复合酶制剂组和联合添加组肉鸡的21日龄体重、1~21日龄ADFI和ADG、1~42日龄ADFI和ADG显著(P<0.05)高于复合微生态制剂组。复合微生态制剂组肉鸡的1~21日龄和22~42日龄F/G显著(P<0.05)低于复合酶制剂组和联合添加组,1~42日龄F/G显著(P<0.05)低于复合酶制剂组。②与对照组相比,3个试验组肉鸡的21日龄和42日龄血清TG含量均显著(P<0.05)降低。联合添加组肉鸡的42日龄血清TP含量显著(P<0.05)高于对照组和复合微生态制剂组。复合酶制剂组肉鸡的42日龄血清GLU含量显著(P<0.05)高于其他3组。③各组肉鸡的21[Objective]This study was conducted to assess the effects of dietary supplementation of compound enzyme and probiotic preparations on growth performance,serum biochemical indices and antioxidant capacity,and small intestinal morphological development of AA broilers.[Method]A total of 480 one-day-old male AA broilers with similar weights were randomly assigned into a control group and 3 treatment groups(T1,T2,T3).The control group fed a corn-soybean meal based diet,while the T1,T2 and T3 groups fed the same basal diets containing 0.2 g/kg compound enzyme preparation,1 g/kg compound probiotic preparation,and 0.2 g/kg compound enzyme preparation+1 g/kg compound probiotic preparation,respectively.There were 6 replicates in each group and 20 broilers in each replicate.The feeding trial lasted for 42 days.The average daily feed intake(ADFI),average daily gain(ADG),and feed to gain ratio(F/G)at different growth stages(1-21 days of age,22-42 days of age,1-42 days of age)were calculated.On the 21st and 42nd day of the trial,blood samples were collected to determine the serum biochemical indices and antioxidant capacity.Samples of duodenum,jejunum and ileum were collected to prepare tissue sections.The villus height(VH)and crypt depth(CD)of each small intestinal segment was observed after HE staining,and the ratio of villus height to crypt depth(V/C)was calculated.[Result]①Compared with the control group,significantly(P<0.05)higher weight at 21 days of age,ADFI and ADG at 1-21 days of age,and ADG at 1-42 days of age were observed in T1 and T3 groups;T1 group had significantly(P<0.05)increased ADFI at 22-42 days and 1-42 days of age,and had significantly(P<0.05)decreased F/G at 1-42 days of age;the F/G at 1-21 days,22-42 days and 1-42 days of age in T2 group was significantly(P<0.05)reduced;T3 group had significantly(P<0.05)decreased F/G at 22-42 days and 1-42 days of age.The weight at 21 days of age as well as the ADFI and ADG at 1-21 days and 1-42 days of age in T1 and T3 groups were significantly(P<0.05)higher than tho

关 键 词：肉鸡 复合酶制剂 复合微生态制剂 生长性能 血清生化 小肠形态发育 

分 类 号：S831.925[农业科学—畜牧学] S816.7[农业科学—畜牧兽医]